Cheng Yuxuan, Ru Jing, Feng Chaobo, Liu Xiaohao, Zeng Hua, Tan Shuo, Chen Xi, Chen Feng, Lu Bing-Qiang
Suzhou First People's Hospital, School of Medicine, Anhui University of Science and Technology, 168 Taifeng Street, Shannan New District, Huainan 232000, Anhui, P. R. China.
Center for Orthopaedic Science and Translational Medicine, Department of Orthopedic, Spinal Pain Research Institute, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200072, P. R. China.
ACS Omega. 2024 Apr 6;9(15):17334-17343. doi: 10.1021/acsomega.3c10427. eCollection 2024 Apr 16.
The constituent ions of calcium phosphate in body fluids are in the supersaturated state and tend to form minerals physiologically or pathologically. Inorganic pyrophosphate (PPi) has been considered as one of the most important inhibitors against the formation of calcium phosphate minerals. However, serum PPi concentrations in humans are maintained at a level of several μmol/L, and its effectiveness and mechanism for mineralization inhibition remain ambiguous. Therefore, this work studied the mineralization process in an aqueous solution, explored the effective inhibitory concentration of PPi by titration, and characterized the species during the reactions. We find that PPi at a normal serum concentration does not inhibit mineralization significantly. Such a conclusion was further confirmed in the PPi-added serum. This work indicates that PPi may not be a major direct inhibitor of mineralization in serum and possibly functions via alternative mechanisms.
体液中磷酸钙的组成离子处于过饱和状态，易于在生理或病理状态下形成矿物质。无机焦磷酸（PPi）被认为是抑制磷酸钙矿物质形成的最重要抑制剂之一。然而，人体血清中PPi的浓度维持在几微摩尔/升的水平，其抑制矿化的有效性和机制仍不明确。因此，本研究在水溶液中研究了矿化过程，通过滴定探索了PPi的有效抑制浓度，并对反应过程中的物种进行了表征。我们发现，正常血清浓度的PPi对矿化没有明显抑制作用。这一结论在添加了PPi的血清中得到了进一步证实。本研究表明，PPi可能不是血清中矿化的主要直接抑制剂，可能通过其他机制发挥作用。
