Chen Lin, Bian Lusen, Ma Qinghua, Li Ying, Wang Xinghong, Liu Yunpeng
National Permanent Scientific Research Base for Warm Temperate Zone Forestry of Jiulong Mountain, Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 102300, China.
State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Hortic Res. 2024 Feb 23;11(4):uhae049. doi: 10.1093/hr/uhae049. eCollection 2024 Apr.
Grafting is a traditional and significant strategy to suppress soil-borne diseases, such as the crown gall disease caused by tumorigenic and . Root exudates and the rhizosphere microbiome play critical roles in controlling crown gall disease, but their roles in suppressing crown gall disease in grafted plants remain unclear. Here, disease-susceptible cherry rootstock 'Gisela 6' and disease-resistant cherry rootstock 'Haiying 1' were grafted onto each other or self-grafted. The effect of their root exudates on the soil microbiome composition and the abundance of pathogenic were studied. Grafting onto the disease-resistant rootstock helped to reduce the abundance of pathogenic , accompanied by altering root exudation, enriching potential beneficial bacteria, and changing soil function. Then, the composition of the root exudates from grafted plants was analyzed and the potential compounds responsible for decreasing pathogenic abundance were identified. Based on quantitative measurement of the concentrations of the compounds and testing the impacts of supplied pure chemicals on abundance and chemotaxis of pathogenic and potential beneficial bacteria, the decreased valine in root exudates of the plant grafted onto resistant rootstock was found to contribute to decreasing abundance, enriching some potential beneficial bacteria and suppressing crown gall disease. This study provides insights into the mechanism whereby grafted plants suppress soil-borne disease.
嫁接是一种传统且重要的防治土传病害的策略，比如由致瘤性[具体病菌未给出]引起的冠瘿病。根系分泌物和根际微生物群落在控制冠瘿病方面发挥着关键作用，但它们在嫁接植物中抑制冠瘿病的作用仍不清楚。在此，将感病樱桃砧木‘吉塞拉6号’和抗病樱桃砧木‘海樱1号’相互嫁接或自嫁接。研究了它们的根系分泌物对土壤微生物群落组成和致病[具体病菌未给出]丰度的影响。嫁接到抗病砧木上有助于降低致病[具体病菌未给出]的丰度，同时改变根系分泌物、富集潜在有益细菌并改变土壤功能。然后，分析了嫁接植物根系分泌物的组成，并鉴定了导致致病[具体病菌未给出]丰度降低的潜在化合物。基于对这些化合物浓度的定量测量以及测试所供应的纯化学品对致病[具体病菌未给出]和潜在有益细菌的丰度及趋化性的影响，发现嫁接到抗病砧木上的植物根系分泌物中缬氨酸的减少有助于降低[具体病菌未给出]的丰度、富集一些潜在有益细菌并抑制冠瘿病。本研究为嫁接植物抑制土传病害的机制提供了见解。
