Rembiszewska A, Brynczak A
Folia Histochem Cytobiol. 1985;23(3):159-63.
The steroidogenic activity of the ovarian surface epithelium in various steps of biosynthesis of the sex steroids was investigated on 105 specimen of the ovaries obtained during laparotomy in the Department of Obstetrics and Gynecology of Postgraduate Medical School. The slices of the ovary were incubated according to the method elaborated by Levy et al. The studies were performed during the follicular and luteal phase of the menstrual phase. For the determination of the activity of delta 5-3-beta-hydroxysteroid dehydrogenase pregnenolone and dehydroepiandrosterone were used. For the determination of 17 beta-hydroxysteroid dehydrogenase androstenedione and testosterone were applied. The results obtained indicate, that the cells of the human surface epithelium have the enzyme system necessary for the different stages of steroidogenesis. However the results obtained do not prove that steroidogenic precursors are synthesized de novo in cells of the surface epithelium.
在研究生医学院妇产科剖腹手术过程中获取的105份卵巢标本上，研究了卵巢表面上皮在性类固醇生物合成各个步骤中的类固醇生成活性。卵巢切片按照Levy等人阐述的方法进行孵育。研究在月经周期的卵泡期和黄体期进行。使用孕烯醇酮和脱氢表雄酮来测定δ5-3-β-羟基类固醇脱氢酶的活性。应用雄烯二酮和睾酮来测定17-β-羟基类固醇脱氢酶的活性。所得结果表明，人表面上皮细胞具有类固醇生成不同阶段所需的酶系统。然而，所得结果并未证明类固醇生成前体是在表面上皮细胞中从头合成的。
