Polysaccharides Enhance the Preventive Efficacy of Heat-Inactivated WX-94 against High-Fat-High-Sucrose-Induced Liver Injury and Gut Dysbacteriosis.

() is an efficacious probiotic that could reduce inflammation and prevent metabolic disorders. Here, we innovatively found that polysaccharides (PKP) promoted proliferation and increased stability of WX-94 (a probiotic strain showing anti-inflammation potentials) in simulated digestive fluids . PKP was composed of galactose, glucose, mannose, and arabinose. The cell-free supernatant extracted from cultured with PKP increased ABTS•+, DPPH•, and FRAP scavenging capacities compared with the supernatant of the medium without PKP and increased metabolites with health-promoting activities, e.g., 3-phenyllactic acid, indole-3-lactic acid, indole-3-carbinol, and propionic acid. Moreover, PKP enhanced alleviating effects of heat-inactivated on high-fat-high-sucrose-induced liver injury in rats via reducing inflammation and regulating expressions of protein and genes involved in fatty acid metabolism (such as HIF1-α, FAβO, CPT1, and AMPK) and fatty acid profiles in liver. Such benefits correlated with its prominent effects on enriching and short-chain fatty acids while reducing , and . Our work provides novel insights into the probiotic property of PKP and emphasizes the great potential of the inactivated cultured with PKP in contracting unhealthy diet-induced liver dysfunctions and gut dysbacteriosis.