Huang Xiaohan, Song Qiaoqiao, Guo Shuqi, Fei Qiang
School of Chemical Engineering and Technology, Xi'an Jiaotong University, Xi'an, 710049, China.
Shaanxi Key Laboratory of Energy Chemical Process Intensification, Xi'an Jiaotong University, Xi'an, 710049, China.
Bioresour Bioprocess. 2022 Dec 12;9(1):126. doi: 10.1186/s40643-022-00614-3.
As a promising industrial microorganism, methylotroph is capable of using methane or methanol as the sole carbon source natively, which has been utilized in the biosynthesis of various bioproducts. However, the relatively low efficiency of carbon conversion has become a limiting factor throughout the development of methanotrophic cell factories due to the unclear genetic background. To better highlight their advantages in methane or methanol-based biomanufacturing, some metabolic engineering strategies, including upstream transcription regulation projects, are being popularized in methylotrophs. In this review, several strategies of transcription regulations applied in methylotrophs are summarized and their applications are discussed and prospected.
作为一种有前景的工业微生物，甲基营养菌能够天然地利用甲烷或甲醇作为唯一碳源，已被用于各种生物产品的生物合成。然而，由于遗传背景不明确，碳转化效率相对较低已成为甲烷营养型细胞工厂整个发展过程中的一个限制因素。为了更好地突出它们在基于甲烷或甲醇的生物制造中的优势，一些代谢工程策略，包括上游转录调控项目，正在甲基营养菌中得到推广。在这篇综述中，总结了应用于甲基营养菌的几种转录调控策略，并对其应用进行了讨论和展望。
