Sun Yue, Wu Shaoting, Fu Xiao, Lai Chongde, Guo Daoyi
College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, 330045, China.
Key Laboratory of Organo-Pharmaceutical Chemistry, Gannan Normal University, Ganzhou, 341000, Jiangxi Province, China.
Bioresour Bioprocess. 2022 Mar 21;9(1):29. doi: 10.1186/s40643-022-00521-7.
τ-Cadinol is a sesquiterpene that is widely used in perfume, fine chemicals and medicines industry. In this study, we established a biosynthetic pathway for the first time in engineered Escherichia coli for production of τ-cadinol from simple carbon sources. Subsequently, we further improved the τ-cadinol production to 35.9 ± 4.3 mg/L by optimizing biosynthetic pathway and overproduction of rate-limiting enzyme IdI. Finally, the titer was increased to 133.5 ± 11.2 mg/L with a two-phase organic overlay-culture medium system. This study shows an efficient method for the biosynthesis of τ-cadinol in E. coli with the heterologous hybrid MVA pathway.
τ-杜松醇是一种倍半萜烯，广泛应用于香水、精细化学品和医药行业。在本研究中，我们首次在工程化大肠杆菌中建立了一条生物合成途径，用于从简单碳源生产τ-杜松醇。随后，我们通过优化生物合成途径和过量表达限速酶IdI，将τ-杜松醇的产量进一步提高到35.9±4.3mg/L。最后，采用两相有机覆盖培养基系统，将产量提高到133.5±11.2mg/L。本研究展示了一种利用异源杂交MVA途径在大肠杆菌中高效生物合成τ-杜松醇的方法。
