工程化大肠杆菌中τ-杜松醇的从头生物合成。
De novo biosynthesis of τ-cadinol in engineered Escherichia coli.
作者信息
Sun Yue, Wu Shaoting, Fu Xiao, Lai Chongde, Guo Daoyi
机构信息
College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, 330045, China.
Key Laboratory of Organo-Pharmaceutical Chemistry, Gannan Normal University, Ganzhou, 341000, Jiangxi Province, China.
出版信息
Bioresour Bioprocess. 2022 Mar 21;9(1):29. doi: 10.1186/s40643-022-00521-7.
τ-Cadinol is a sesquiterpene that is widely used in perfume, fine chemicals and medicines industry. In this study, we established a biosynthetic pathway for the first time in engineered Escherichia coli for production of τ-cadinol from simple carbon sources. Subsequently, we further improved the τ-cadinol production to 35.9 ± 4.3 mg/L by optimizing biosynthetic pathway and overproduction of rate-limiting enzyme IdI. Finally, the titer was increased to 133.5 ± 11.2 mg/L with a two-phase organic overlay-culture medium system. This study shows an efficient method for the biosynthesis of τ-cadinol in E. coli with the heterologous hybrid MVA pathway.
τ-杜松醇是一种倍半萜烯，广泛应用于香水、精细化学品和医药行业。在本研究中，我们首次在工程化大肠杆菌中建立了一条生物合成途径，用于从简单碳源生产τ-杜松醇。随后，我们通过优化生物合成途径和过量表达限速酶IdI，将τ-杜松醇的产量进一步提高到35.9±4.3mg/L。最后，采用两相有机覆盖培养基系统，将产量提高到133.5±11.2mg/L。本研究展示了一种利用异源杂交MVA途径在大肠杆菌中高效生物合成τ-杜松醇的方法。
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