在代谢工程大肠杆菌中从葡萄糖生物合成橙花醇。
Biosynthesis of nerol from glucose in the metabolic engineered Escherichia coli.
机构信息
Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Key Laboratory of Industrial Microbiology, Hubei Collaborative Innovation Center of Industrial Fermentation, Hubei University of Technology, Wuhan 430068, China.
Key Laboratory of Organo-Pharmaceutical Chemistry, Jiangxi Province, Gannan Normal University, Ganzhou 341000, China.
出版信息
Bioresour Technol. 2019 Sep;287:121410. doi: 10.1016/j.biortech.2019.121410. Epub 2019 May 2.
In this study, nerol was biosynthesized in the metabolic engineered Escherichia coli from glucose for the first time. Firstly, the truncated neryl diphosphate synthase gene tNDPS1 was expressed that catalyzes isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) to form neryl diphosphate (NPP), and then the nerol synthase gene GmNES was co-expressed to synthesize the final product nerol from NPP. The engineered strain LZ001 accumulated 0.053 ± 0.015 mg/L of nerol. Secondly, the IDI1, MVD1, ERG8, ERG12, tHMG1 and ERG13 were co-expressed to increase the supply of IPP and DMAPP. Finally, the heterologous ERG10 gene was overexpressed, and the recombinant strain LZ005 produced 1.564 ± 0.102 mg/L of nerol in shaking-flask culture, which represents a 29.51-fold increase over LZ001 strain. This study shows the novel method for the biosynthesis of nerol and provides new metabolic engineering strategy for the production of terpenoids.
在这项研究中，首次通过代谢工程大肠杆菌从葡萄糖生物合成了橙花醇。首先，表达了截断的香叶基二磷酸合酶基因 tNDPS1，该酶催化异戊烯二磷酸（IPP）和二甲基烯丙基二磷酸（DMAPP）形成香叶基二磷酸（NPP），然后共表达橙花醇合酶基因 GmNES 以从 NPP 合成最终产物橙花醇。工程菌株 LZ001 积累了 0.053±0.015mg/L 的橙花醇。其次，共表达 IDI1、MVD1、ERG8、ERG12、tHMG1 和 ERG13 以增加 IPP 和 DMAPP 的供应。最后，过表达异源 ERG10 基因，重组菌株 LZ005 在摇瓶培养中产生 1.564±0.102mg/L 的橙花醇，比 LZ001 菌株增加了 29.51 倍。本研究展示了橙花醇生物合成的新方法，并为萜类化合物的生产提供了新的代谢工程策略。
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