Lessard Lola, Saugues Audrey, Gondin Julien, Mounier Rémi, Kneppers Anita
Institut NeuroMyoGène, Physiopathologie et Génétique du Neurone et du Muscle, Université Claude Bernard Lyon 1, CNRS UMR5261, INSERM U1315, Lyon, France.
Methods Mol Biol. 2024 Apr 23. doi: 10.1007/7651_2024_540.
Adult skeletal muscle stem cells (MuSC) are the regenerative precursors of myofibers and also have an important role in myofiber growth, adaptation, and maintenance by fusing to the myofibers-a process referred to as "myonuclear accretion." Due to a focus on MuSC function during regeneration, myofibers remain a largely overlooked component of the MuSC niche influencing MuSC fate. Here, we describe a method to directly measure the rate of myonuclear accretion in vitro and in vivo using ethynyl-2'-deoxyuridine (EdU)-based tracing of MuSC progeny. This method supports the dissection of MuSC intrinsic and myofiber-derived factors influencing myonuclear accretion as an alternative fate of MuSCs supporting myofiber homeostasis and plasticity.
成体骨骼肌干细胞（MuSC）是肌纤维的再生前体细胞，并且通过与肌纤维融合（这一过程称为“肌核增加”）在肌纤维生长、适应和维持中发挥重要作用。由于在再生过程中对MuSC功能的关注，肌纤维在很大程度上仍是影响MuSC命运的MuSC微环境中被忽视的组成部分。在这里，我们描述了一种使用基于乙炔基-2'-脱氧尿苷（EdU）追踪MuSC后代的方法，直接在体外和体内测量肌核增加的速率。这种方法有助于剖析影响肌核增加的MuSC内在因素和肌纤维衍生因素，肌核增加是MuSC支持肌纤维稳态和可塑性的另一种命运。
