Namdar Nazila Zeynali, Roufegarinejad Leila, Alizadeh Ainaz, Asefi Narmela, Jafari Seid Mahdi, Sarabandi Khashayar
Department of Food Science and Technology, Islamic Azad University, Tabriz Branch, Tabriz, Iran.
Department of Food Materials & Process Design Engineering, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Bioresour Bioprocess. 2023 Dec 1;10(1):87. doi: 10.1186/s40643-023-00709-5.
This study aimed to produce bioactive peptides from navy-bean protein with alcalase and pepsin enzymes (30-300 min) and to load them into a nanoliposome system to stabilize and improve their bioavailability. The degree of hydrolysis and biological activities (scavenging of DPPH, OH, and ABTS free radicals, reducing power, and chelating metal ions) of navy-bean protein were affected by the type of enzyme and hydrolysis time. The average particle size (83-116 nm), PDI (0.23-0.39), zeta potential (- 13 to - 20 mV), and encapsulation efficiency (80-91%) of nanoliposomes were influenced by the type and charge of peptides. The storage temperature and the type of loaded peptide greatly affected the physical stability of nanocarriers and maintaining EE during storage. The FTIR results suggested the effect of enzymatic hydrolysis on the secondary structures of protein and the effective placement of peptides inside polar-regions and the phospholipid monolayer membrane. SEM images showed relatively uniform-sized particles with irregular structures, which confirmed the results of DLS. The antioxidant activity of primary peptides affected the free radical scavenging of loaded nanoliposomes. Liposomes loaded with navy-bean peptides can be used as a health-giving formula in enriching all kinds of drinks, desserts, confectionery products, etc.
本研究旨在利用碱性蛋白酶和胃蛋白酶（作用30 - 300分钟）从海军豆蛋白中制备生物活性肽，并将其载入纳米脂质体系统以稳定并提高其生物利用度。海军豆蛋白的水解程度和生物活性（清除DPPH、OH和ABTS自由基、还原能力以及螯合金属离子）受酶的类型和水解时间影响。纳米脂质体的平均粒径（83 - 116纳米）、多分散指数（0.23 - 0.39）、zeta电位（-13至-20毫伏）和包封效率（80 - 91%）受肽的类型和电荷影响。储存温度和负载肽的类型极大地影响了纳米载体的物理稳定性以及储存期间包封效率的维持。傅里叶变换红外光谱结果表明酶解对蛋白质二级结构的影响以及肽在极性区域和磷脂单分子膜内的有效定位。扫描电子显微镜图像显示颗粒大小相对均匀但结构不规则，这证实了动态光散射的结果。初级肽的抗氧化活性影响了负载纳米脂质体对自由基的清除。负载海军豆肽的脂质体可用作一种有益健康的配方，用于强化各类饮品、甜点、糖果制品等。
