Islam Md Serajul, Hongxin Wang, Admassu Habtamu, Noman Anwar, Ma Chaoyang, An Wei Fu
State key Laboratory of Food Science and Technology Jiangnan University Wuxi Jiangsu province 214122 China.
National Engineering Research Center for Functional Food Jiangnan University Wuxi Jiangsu province 214122 China.
Food Sci Nutr. 2021 Jun 24;9(8):4031-4047. doi: 10.1002/fsn3.1903. eCollection 2021 Aug.
Grass turtle muscle was hydrolyzed with papain enzyme to produce protein hydrolysate (PH) and the degree of hydrolysis (DH) was determined. Under optimal conditions, the highest DH was 19.52% and the yield was recorded as 17.26%. Protein content of the hydrolysates was ranged from 73.35% to 76.63%. Total amino acids were more than 96.77% for each PH. The PH obtained at DH 19.52% achieved excellent solubility and emulsifying activity which were 95.56% and 108.76 m/g, respectively at pH 6. Foam capacity amounted 100% in PH of DH 19.52% at pH 2, and water-holding capacity was 4.38 g/g. The antioxidant activity showed the strongest hydroxyl radical scavenging activity (95.25%), ABTS (84.88%), DPPH (75.89%), iron chelating (63.25%), and cupper chelating (66.90%) at DH 11.96%, whereas reducing power (0.88) at DH 19.52%. Thus, the findings indicated that utilization of grass turtle muscle protein hydrolysate is a potential alternative protein resource to improve the nutritional and functional properties in food ingredients and product formulations.
用木瓜蛋白酶水解草龟肌肉以产生蛋白水解物（PH），并测定水解度（DH）。在最佳条件下，最高水解度为19.52%，产率记录为17.26%。水解物的蛋白质含量在73.35%至76.63%之间。每种蛋白水解物的总氨基酸含量均超过96.77%。在pH值为6时，水解度为19.52%的蛋白水解物具有优异的溶解性和乳化活性，分别为95.56%和108.76 m/g。在pH值为2时，水解度为19.52%的蛋白水解物的泡沫容量为100%，持水能力为4.38 g/g。抗氧化活性方面，在水解度为11.96%时表现出最强的羟基自由基清除活性（95.25%）、ABTS（84.88%）、DPPH（75.89%）、铁螯合（63.25%）和铜螯合（66.90%），而在水解度为19.52%时还原力为0.88。因此，研究结果表明，利用草龟肌肉蛋白水解物是一种潜在的替代蛋白质资源，可改善食品成分和产品配方中的营养和功能特性。
