The First Clinical Medical College, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
The Sixth Department of Acupuncture and Moxibustion, The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150040.
Zhen Ci Yan Jiu. 2024 Apr 25;49(4):415-423. doi: 10.13702/j.1000-0607.20230019.
To explore the mechanism of core points in acupuncture and moxibustion treatment for epilepsy by using data mining technique, so as to provide a reference for clinical practice and experimental research.
The data comes from relevant documents collected from CNKI, Wanfang, SinoMed, VIP, PubMed, Embase, Cochrane Library, EBSCO, Web of Science databases. The selected acupoints were analyzed in descriptive statistics, high-frequency acupoints group and core acupoint prescription. Further, potential target mining, "core acupoint prescription-target-epilepsy" network construction, protein-protein interactions (PPI) network establishment and core target extraction, gene ontology (GO) and KEGG gene enrichment analysis of the core acupoint prescription were carried out to predict its anti-epileptic potential mechanism.
A total of 122 acupoint prescriptions were included. The core acupoint prescriptions were Baihui (GV20), Hegu (LI4), Neiguan (PC6), Shuigou (GV26) and Taichong (LR3). 277 potential targets were identified, among which 134 were shared with epilepsy. The core targets were extracted by PPI network topology analysis, including signal transducer and activator of transcription 3, tumor necrosis factor (TNF), interleukin (IL)-6, protein kinase B1, c-Jun N-terminal kinase, brain-derived neurotrophic factor, tumor protein 53, vascular endothelial growth factor A, Caspase-3, epidermal growth factor receptor, etc. The main anti-epileptic pathways of the core acupoints were predicted by KEGG enrichment, including lipid and atherosclerosis, neurodegeneration, phosphatidylinositol-3-kinase/protein B kinase signaling pathway, mitogen-activated protein kinase signaling pathway, cyclic adenosine monophosphate signaling pathway, TNF signaling pathway, IL-17 signaling pathway, hypoxia-inducible factor-1 signaling pathway, apoptosis, etc., involving neuronal death, synaptic plasticity, oxidative stress, inflammation and other related biological process.
The core acupoint prescription of acupuncture and moxibustion intervention for epilepsy can act on multiple targets and multiple pathways to exert anti-epileptic effects, which can provide a theoretical basis for further clinical application and mechanism research.
运用数据挖掘技术探讨针灸治疗癫痫的核心腧穴机制，为临床实践和实验研究提供参考。
资料来源于中国知网、万方、中国生物医学文献服务系统、维普、PubMed、Embase、Cochrane 图书馆、EBSCO、Web of Science 数据库中针灸治疗癫痫的相关文献，对选用的腧穴进行描述性统计、高频腧穴组及核心腧穴处方分析，进一步进行潜在靶标挖掘、“核心腧穴处方-靶标-癫痫”网络构建、蛋白质-蛋白质相互作用（PPI）网络建立及核心靶标提取，对核心腧穴处方进行基因本体（GO）和京都基因与基因组百科全书（KEGG）基因富集分析，预测其抗癫痫的潜在作用机制。
共纳入 122 首处方，核心腧穴处方为百会（GV20）、合谷（LI4）、内关（PC6）、水沟（GV26）、太冲（LR3）。共筛选出 277 个潜在靶标，其中 134 个与癫痫共有。通过 PPI 网络拓扑分析提取核心靶标，包括信号转导和转录激活因子 3、肿瘤坏死因子（TNF）、白细胞介素（IL）-6、蛋白激酶 B1、c-Jun N 末端激酶、脑源性神经营养因子、肿瘤蛋白 53、血管内皮生长因子 A、Caspase-3、表皮生长因子受体等。通过 KEGG 富集预测核心腧穴的主要抗癫痫通路，包括脂类与动脉粥样硬化、神经退行性疾病、磷脂酰肌醇 3-激酶/蛋白激酶 B 信号通路、丝裂原活化蛋白激酶信号通路、环磷酸腺苷信号通路、TNF 信号通路、IL-17 信号通路、缺氧诱导因子-1 信号通路、细胞凋亡等，涉及神经元死亡、突触可塑性、氧化应激、炎症等相关生物学过程。
针灸治疗癫痫的核心腧穴处方可以通过多个靶标和多条通路发挥抗癫痫作用，为进一步的临床应用和机制研究提供理论依据。
