Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
The Department of Cardiology, The Heart Centre, Copenhagen University Hospital, Copenhagen, Denmark.
Int J Legal Med. 2024 Sep;138(5):1787-1790. doi: 10.1007/s00414-024-03232-0. Epub 2024 Apr 23.
It is pivotal to avoid cross-sample contamination in forensic genetic laboratories and optimal cleaning protocols for the removal of DNA are essential. A survey was performed, and ten forensic genetic laboratories shared their cleaning protocols in pre-PCR and post-PCR laboratories. The cleaning frequencies on different surface areas were somewhat similar, whereas none of the laboratories used the same cleaning reagents. Therefore, the efficiencies of the cleaning protocol utilised were tested and compared. The results showed that freshly made household bleach and Virkon® removed all amplifiable DNA from the surfaces, whereas DNA AWAY™ and the disinfection reagents ethanol, isopropanol, and ChemGene HLDL did not.
在法医遗传实验室中，避免交叉样品污染至关重要，因此去除 DNA 的最佳清洁方案必不可少。我们进行了一项调查，有 10 家法医遗传实验室分享了他们在 PCR 前和 PCR 后实验室的清洁方案。不同表面区域的清洁频率有些相似，但没有一个实验室使用相同的清洁试剂。因此，我们测试并比较了所使用清洁方案的效率。结果表明，新鲜的家用漂白剂和 Virkon®可以从表面去除所有可扩增的 DNA，而 DNA AWAY™和消毒试剂乙醇、异丙醇和 ChemGene HLDL 则不能。
