Key Laboratory of Plant Carbon Capture, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China.
Methods Mol Biol. 2024;2790:213-226. doi: 10.1007/978-1-0716-3790-6_11.
Canopy photosynthesis (A), rather than leaf photosynthesis, is critical to gaining higher biomass production in the field because the daily or seasonal integrals of A correlate with the daily or seasonal integrals of biomass production. The canopy photosynthesis and transpiration measurement system (CAPTS) was developed to enable measurement of canopy photosynthetic CO uptake, transpiration, and respiration rates. CAPTS continuously records the CO concentration, water vapor concentration, air temperature, air pressure, air relative humidity, and photosynthetic photon flux density (PPFD) inside the chamber, which can be used to derive CO and HO fluxes of a canopy covered by the chamber. This system can also be used to measure the fluxes of greenhouse gases when integrating with CH and NO analyzers. Here, we describe the protocol for using CAPTS to perform experiments on rice (Oryza sativa L.) in paddy field, wheat (Triticum aestivum L.) in upland field, and tobacco (Nicotiana tabacum L.) in pots.
冠层光合作用（A），而不是叶片光合作用，对获得田间更高的生物量生产至关重要，因为 A 的日或季节积分与生物量生产的日或季节积分相关。冠层光合作用和蒸腾测量系统（CAPTS）的开发是为了能够测量冠层光合 CO 吸收、蒸腾和呼吸速率。CAPTS 连续记录室内的 CO 浓度、水汽浓度、空气温度、气压、空气相对湿度和光合光子通量密度（PPFD），可用于推导覆盖室内的冠层的 CO 和 HO 通量。当与 CH 和 NO 分析仪集成时，该系统还可用于测量温室气体通量。在这里，我们描述了使用 CAPTS 在稻田中的水稻（Oryza sativa L.）、旱地中的小麦（Triticum aestivum L.）和盆中的烟草（Nicotiana tabacum L.）上进行实验的方案。
