Song Qingfeng, Zhu Xin-Guang
National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China.
State Key Laboratory of Hybrid Rice, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
Methods Mol Biol. 2018;1770:69-81. doi: 10.1007/978-1-4939-7786-4_4.
Canopy photosynthesis (A), rather than leaf photosynthesis, is critical to gaining higher biomass production in the field because the daily or seasonal integrals of A correlate with the daily or seasonal integrals of biomass production. The canopy photosynthesis and transpiration measurement system (CAPTS) was developed to enable measurement of canopy photosynthetic CO uptake, transpiration, and respiration rates. CAPTS continuously records the CO concentration, water vapor concentration, air temperature, air pressure, air relative humidity, and photosynthetic photon flux density (PPFD) inside the chamber, which can be used to derive CO and HO fluxes of a canopy covered by the chamber. Here we describe the protocol of using CAPTS to perform experiments on rice (Oryza sativa L.) in paddy field, wheat (Triticum aestivum L.) in upland field, and tobacco (Nicotiana tabacum L.) in pots.
冠层光合作用（A）而非叶片光合作用，对于在田间获得更高生物量产量至关重要，因为A的日积分或季节积分与生物量产量的日积分或季节积分相关。冠层光合作用和蒸腾测量系统（CAPTS）的开发是为了能够测量冠层光合CO吸收、蒸腾和呼吸速率。CAPTS连续记录室内的CO浓度、水汽浓度、气温、气压、空气相对湿度和光合光子通量密度（PPFD），这些可用于推导被该室覆盖的冠层的CO和HO通量。在此，我们描述了使用CAPTS在稻田水稻（Oryza sativa L.）、旱地小麦（Triticum aestivum L.）和盆栽烟草（Nicotiana tabacum L.）上进行实验的方案。
