溶血素对深海生物的致病性至关重要。
Haemolysins are essential to the pathogenicity of deep-sea .
作者信息
Wang Yujian, Luo Jingchang, Zhao Yan, Zhang Jian, Guan Xiaolu, Sun Li
机构信息
CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China.
Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao 266237, China.
出版信息
iScience. 2024 Mar 26;27(5):109558. doi: 10.1016/j.isci.2024.109558. eCollection 2024 May 17.
is an emerging foodborne pathogen that produces VFH ( hemolysin) and δVFH (delta- hemolysin). The function of δVFH is unclear. Currently, no pathogenic . from deep sea has been reported. In this work, a deep-sea . isolate (V13) was examined for pathogenicity. V13 was most closely related to ATCC 33809, a human isolate, but possessed 262 unique genes. V13 caused lethal infection in fish and induced pyroptosis involving activation of the NLRP3 inflammasome, caspase 1 (Casp1), and gasdermin D (GSDMD). V13 defective in VFH or VFH plus δVFH exhibited significantly weakened cytotoxicity. Recombinant δVFH induced NLRP3-Casp1-GSDMD-mediated pyroptosis in a manner that depended on K efflux and intracellular Ca accumulation. δVFH bound several plasma membrane lipids, and these bindings were crucial for δVFH cytotoxicity. Together these results provided new insights into the function of δVFH and the virulence mechanism of .
是一种新兴的食源性病原体，可产生VFH（溶血素）和δVFH（δ-溶血素）。δVFH的功能尚不清楚。目前，尚未有来自深海的致病性[病原体名称未明确]的报道。在这项研究中，对一株深海[病原体名称未明确]分离株（V13）进行了致病性检测。V13与人类分离株[具体菌株名称未明确]ATCC 33809关系最为密切，但拥有262个独特基因。V13在鱼类中引起致命感染，并诱导细胞焦亡，涉及NLRP3炎性小体、半胱天冬酶1（Casp1）和gasdermin D（GSDMD）的激活。VFH或VFH加δVFH缺陷的V13表现出明显减弱的细胞毒性。重组δVFH以依赖钾离子外流和细胞内钙积累的方式诱导NLRP3 - Casp1 - GSDMD介导的细胞焦亡。δVFH与几种质膜脂质结合，这些结合对于δVFH的细胞毒性至关重要。这些结果共同为δVFH的功能和[病原体名称未明确]的毒力机制提供了新的见解。
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