The study investigated the occurrence of antimicrobial resistance genes and virulence determinants in species recovered from different freshwater sheds in rustic milieu. A total of 118 isolates comprising (n=41), (n=40) and (n=37) was identified by amplification of , and genes. The amplification of virulence genes indicated that . (, , , , and ) genes were detected in 12.5%, 32.5%, 45%, 37.5% and 10% respectively. . genes (, and ) were harboured in 48.8%, 14.6% and 19.5% isolates congruently. The other virulence genes that include and were observed in 63.1% and 29% of isolates belonging to . . With the exceptions of imipenem, meropenem and ciprofloxacin, most isolates exhibited more than 50% resistance to antibiotics. The antimicrobial resistance was more prevalent for polymyxin B (100%), azithromycin (100%) and least in ciprofloxacin (16.1%). Multiple antibiotic resistance index range was 0.3 and 0.8 with most isolates showing MARI of 0.8. The TEM, AmpC, GES, IMP, OXA-48 and KPC genes were detected in 53.3%, 42%, 29.6%, 16.6%, 15%, 11.3% and 5.6% of the isolates. Non-beta lactamases such as streptomycin resistance ( and ), gentamicin resistance () and quinolone resistance gene () were found in 5.2%, 44.3%, 26% and 2.8%. Chloramphenicol resistance genes ( and ) were found in 5.2% and 44.3% among the isolates. Our findings reveal the presence of antimicrobial resistance genes and virulent species in aquatic environment which can have potential risk to human and animal's health.