卡西科拉多突叶蛾的核基因组和线粒体基因组：小潜蛾亚科（卷蛾科）的首次组装

Nuclear and mitochondrial genomes of Polylopha cassiicola: the first assembly in Chlidanotinae (Tortricidae).

作者信息

Yang Fangyuan, Cao Li-Jun, Chen Jin-Cui, Song Wei, Yu Yuzhu, Wei Shu-Jun

机构信息

Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.

Guangxi National Qinlian Forest Farm, Guangxi, China.

出版信息

Sci Data. 2024 Apr 23;11(1):419. doi: 10.1038/s41597-024-03255-7.

DOI:10.1038/s41597-024-03255-7

PMID:38653995

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11039721/

Abstract

Tortricidae is one of the largest families in Lepidoptera, including subfamilies of Tortricinae, Olethreutinae, and Chlidanotinae. Here, we assembled the gap-free genome for the subfamily Chlidanotinae using Illumina, Nanopore, and Hi-C sequencing from Polylopha cassiicola, a pest of camphor trees in southern China. The nuclear genome is 302.03 Mb in size, with 36.82% of repeats and 98.4% of BUCSO completeness. The karyotype is 2n = 44 for males. We identified 15412 protein-coding genes, 1052 tRNAs, and 67 rRNAs. We also determined the mitochondrial genome of this species and annotated 13 protein-coding genes, 22 tRNAs, and one rRNA. These high-quality genomes provide valuable information for studying phylogeny, karyotypic evolution, and adaptive evolution of tortricid moths.

摘要

卷蛾科是鳞翅目中最大的科之一，包括卷蛾亚科、小卷蛾亚科和澳卷蛾亚科。在此，我们利用来自中国南方樟树害虫樟翠尺蛾的Illumina、Nanopore和Hi-C测序技术，组装了澳卷蛾亚科的无间隙基因组。核基因组大小为302.03 Mb，重复序列占36.82%，BUSCO完整性为98.4%。雄性的核型为2n = 44。我们鉴定出15412个蛋白质编码基因、1052个tRNA和67个rRNA。我们还测定了该物种的线粒体基因组，并注释了13个蛋白质编码基因、22个tRNA和1个rRNA。这些高质量的基因组为研究卷蛾的系统发育、核型进化和适应性进化提供了有价值的信息。