Zhu Jia-Ying, Wei Shu-Jun, Li Qin-Wen, Yang Song, Li Yong-He
Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, College of Forestry, Southwest Forestry University, Kunming 650224, P.R. China.
Mitochondrial DNA. 2012 Oct;23(5):376-8. doi: 10.3109/19401736.2012.696632. Epub 2012 Jul 18.
The complete nucleotide sequence of the mitochondrial genome of the pine tip moth Rhyacionia leptotubula (Lepidoptera, Tortricidae) was determined. The entire genome is 15,877 bp in length, encoding the standard set of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and an A+T-rich control region. The gene order is typical of Lepidoptera and differs from the insect ancestral type in the location of trnM. All protein-coding sequences start with a typical ATN codon, with the exception of COI, which begins with CGA. All tRNA genes could be folded into the typical cloverleaf secondary structure, except trnS2 lacking of dihydrouridine arm. Additionally, the 7-bp long ATACTAA motif is present in the intergenic space sequence located between trnS1 and nad1, and a long T-stretch with conserved ATAGA motif exists in the A+T-rich region, as found in Lepidoptera. The mitogenome given here provides important molecular information to phylogenetic and population genetic researches.
测定了松梢小卷蛾(Rhyacionia leptotubula,鳞翅目,卷蛾科)线粒体基因组的完整核苷酸序列。整个基因组长度为15,877 bp,编码一套标准的13个蛋白质编码基因、22个tRNA基因、2个rRNA基因以及一个富含A+T的控制区。基因排列顺序是鳞翅目典型的,在trnM的位置上与昆虫祖先类型不同。所有蛋白质编码序列均以典型的ATN密码子起始,但COI除外,它以CGA起始。除了缺少二氢尿嘧啶臂的trnS2外,所有tRNA基因都能折叠成典型的三叶草二级结构。此外,在trnS1和nad1之间的基因间隔序列中存在7 bp长的ATACTAA基序,并且在富含A+T的区域存在一个带有保守ATAGA基序的长T串,这在鳞翅目中也有发现。这里给出的线粒体基因组为系统发育和群体遗传学研究提供了重要的分子信息。