Department of Dermatology and Cutaneous Biology, 6559 Thomas Jefferson University , Philadelphia, PA, USA.
Department of Pharmacology, Physiology and Cancer Biology, 6559 Thomas Jefferson University , Philadelphia, PA, USA.
Clin Chem Lab Med. 2024 Apr 25;62(10):1880-1891. doi: 10.1515/cclm-2023-1245. Print 2024 Sep 25.
Single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) are two emerging research technologies that uniquely characterize gene expression microenvironments on a cellular or subcellular level. The skin, a clinically accessible tissue composed of diverse, essential cell populations, serves as an ideal target for these high-resolution investigative approaches. Using these tools, researchers are assembling a compendium of data and discoveries in healthy skin as well as a range of dermatologic pathophysiologies, including atopic dermatitis, psoriasis, and cutaneous malignancies. The ongoing advancement of single-cell approaches, coupled with anticipated decreases in cost with increased adoption, will reshape dermatologic research, profoundly influencing disease characterization, prognosis, and ultimately clinical practice.
单细胞 RNA 测序 (scRNA-seq) 和空间转录组学 (ST) 是两种新兴的研究技术，可在细胞或亚细胞水平上独特地表征基因表达的微环境。皮肤是一种临床可及的组织，由多种必需的细胞群体组成，是这些高分辨率研究方法的理想目标。使用这些工具，研究人员正在为健康皮肤以及一系列皮肤科病理生理学，包括特应性皮炎、银屑病和皮肤恶性肿瘤，汇编数据和发现。单细胞方法的不断进步，加上随着采用率的提高预期成本的降低，将重塑皮肤科研究，深刻影响疾病特征、预后，最终影响临床实践。
