Joslin-Beth Israel Deaconess Foot Center and The Rongxiang Xu, MD, Center for Regenerative Therapeutics, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.
St John's Institute of Dermatology, School of Basic and Medical Biosciences, King's College London, London, UK.
Exp Dermatol. 2022 May;31(5):655-673. doi: 10.1111/exd.14547. Epub 2022 Mar 4.
Single-cell technologies have revolutionized research in the last decade, including for skin biology. Single-cell RNA sequencing has emerged as a powerful tool allowing the dissection of human disease pathophysiology at unprecedented resolution by assessing cell-to-cell variation, facilitating identification of rare cell populations and elucidating cellular heterogeneity. In dermatology, this technology has been widely applied to inflammatory skin disorders, fibrotic skin diseases, wound healing complications and cutaneous neoplasms. Here, we discuss the available technologies and technical considerations of single-cell RNA sequencing and describe its applications to a broad spectrum of dermatological diseases.
单细胞技术在过去十年中彻底改变了研究，包括皮肤生物学。单细胞 RNA 测序已成为一种强大的工具，通过评估细胞间的变异，以空前的分辨率剖析人类疾病的病理生理学，从而有助于识别罕见的细胞群体并阐明细胞异质性。在皮肤病学中，该技术已广泛应用于炎症性皮肤病、纤维化皮肤病、伤口愈合并发症和皮肤肿瘤。在这里，我们讨论了单细胞 RNA 测序的可用技术和技术考虑因素，并描述了它在广泛的皮肤病中的应用。
