Pawelec G, Newman W, Schwulera U, Wernet P
Cell Immunol. 1985 Apr 15;92(1):31-40. doi: 10.1016/0008-8749(85)90062-0.
Monoclonal antibodies (MoAb) against cell surface determinants were employed to investigate the specificity of natural killer (NK)-like lysis by cloned human effector cells recognizing only K562, only HSB2, or both K562 and HSB2 target cells. MoAb W6/32.HL, TU39, YD1/48.HLK, and anti-Tac failed to inhibit lysis despite the expression of antigens bound by these MoAb on the effector cell surface. MoAb OKT3 moderately (less than or equal to 50%) blocked lysis of K562 and HSB2 targets, whereas MoAb 13.1, which binds T200 molecules, strongly (up to 95%) blocked lysis of K562, but not HSB2, targets. MoAb 13.1 inhibited lysis by clones which killed only K562, as well as lysis by those which killed both HSB2 and K562. In the latter case, however, only lysis of K562 was inhibited. Taken together, these results may suggest the existence of multiple receptor specificities on a single NK-active clone.
利用针对细胞表面决定簇的单克隆抗体（MoAb），研究仅识别K562、仅识别HSB2或同时识别K562和HSB2靶细胞的克隆化人效应细胞进行的自然杀伤（NK）样裂解的特异性。尽管这些MoAb所结合的抗原在效应细胞表面表达，但MoAb W6/32.HL、TU39、YD1/48.HLK和抗Tac均未能抑制裂解。MoAb OKT3对K562和HSB2靶细胞的裂解有中度抑制作用（小于或等于50%），而结合T200分子的MoAb 13.1则强烈抑制（高达95%）K562靶细胞的裂解，但对HSB2靶细胞无抑制作用。MoAb 13.1抑制仅杀伤K562的克隆的裂解，也抑制同时杀伤HSB2和K562的克隆的裂解。然而，在后一种情况下，仅抑制了对K562的裂解。综合这些结果可能提示单个NK活性克隆上存在多种受体特异性。
