Wei Bo, Xu Yan, Tang Cheng, Liu Nancy Q, Li Xuxiang, Yao Qingqiang, Wang Liming
Department of Orthopaedic Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China; Cartilage Regeneration Center, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China.
Department of Orthopaedic Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China; Cartilage Regeneration Center, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China.
Biomater Adv. 2024 Jun;160:213857. doi: 10.1016/j.bioadv.2024.213857. Epub 2024 Apr 12.
Articular cartilage injury impairs joint function and necessitates orthopedic intervention to restore the structure and function of the cartilage. Extracellular matrix (ECM) scaffolds derived from bone marrow mesenchymal stem cells (BMSCs) can effectively promote cell adhesion, proliferation, and chondrogenesis. However, pre-shaped ECM scaffolds have limited applicability due to their poor fit with the irregular surface of most articular cartilage defects. In this study, we fabricated an injectable active ECM hydrogel from autologous BMSCs-derived ECM by freeze-drying, liquid nitrogen milling, and enzymatic digestion. Moreover, our in vitro and in vivo results demonstrated that the prepared hydrogel enhanced chondrocyte adhesion and proliferation, chondrogenesis, cartilage regeneration, and integration with host tissue, respectively. These findings indicate that active ECM components can provide trophic support for cell proliferation and differentiation, restoring the structure and function of damaged cartilage.
关节软骨损伤会损害关节功能，因此需要进行骨科干预以恢复软骨的结构和功能。源自骨髓间充质干细胞（BMSCs）的细胞外基质（ECM）支架可以有效地促进细胞黏附、增殖和软骨形成。然而，预成型的ECM支架与大多数关节软骨缺损的不规则表面贴合性较差，适用性有限。在本研究中，我们通过冷冻干燥、液氮研磨和酶消化，从自体BMSCs衍生的ECM制备了一种可注射的活性ECM水凝胶。此外，我们的体外和体内结果分别表明，制备的水凝胶增强了软骨细胞的黏附、增殖、软骨形成、软骨再生以及与宿主组织的整合。这些发现表明，活性ECM成分可以为细胞增殖和分化提供营养支持，恢复受损软骨的结构和功能。
