de Araujo Fernando Flores, Marcon Raphael Martus, Cristante Alexandre Fogaça, Filho Tarcísio Eloy Pessoa Barros
Instituto de Ortopedia e Traumatologia, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo (IOT-HCFMUSP), São Paulo, SP, Brazil.
Grupo de Cirurgia de Coluna, Laboratório de Investigações Médicas, Instituto de Ortopedia e Traumatologia, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo (IOT-HCFMUSP), São Paulo, SP, Brazil.
Clinics (Sao Paulo). 2024 Apr 23;79:100359. doi: 10.1016/j.clinsp.2024.100359. eCollection 2024.
The aim of this study was to evaluate the GSH effect on functional and histological recovery after experimental spinal cord injury in rats.
Forty Wistar rats were subjected to spinal cord injury through the Multicenter Animal Spinal Cord Injury Study (MASCIS) Impactor system. The rats were sorted and divided into four groups, as follows: Group 1 ‒ Laminectomy and spinal cord injury; Group 2 ‒ Laminectomy, spinal cord injury and Saline Solution (SS) 0.9%; Group 3 ‒ Laminectomy, spinal cord injury, and GSH; and Group 4 ‒ lLaminectomy without spinal cord injury. GSH and SS were administered intraperitoneally. Groups 1 and 4 received no intervention.
The rats were evaluated for locomotor function recovery at seven different times by the Basso, Beattie, and Bresnahan (BBB) scale on days 2, 7, 14, 21, 28, 35, and 42 after the spinal cord injury. On day 42, the rats were sacrificed to analyze the histological findings of the injured spinal cord. In the group submitted to GSH, our experimental study revealed better functional scores on the BBB scale, horizontal ladder scale, and cranial and caudal axon count. The differences found were statistically significant in BBB scores and axonal count analysis.
This study demonstrated that using glutathione in experimental spinal trauma can lead to better functional recovery and improved axonal regeneration rate in Wistar rats submitted to experimental spinal cord injury.
本研究旨在评估谷胱甘肽（GSH）对大鼠实验性脊髓损伤后功能和组织学恢复的影响。
40只Wistar大鼠通过多中心动物脊髓损伤研究（MASCIS）撞击系统遭受脊髓损伤。将大鼠分类并分为四组，如下：第1组 - 椎板切除术和脊髓损伤；第2组 - 椎板切除术、脊髓损伤和0.9%生理盐水（SS）；第3组 - 椎板切除术、脊髓损伤和GSH；第4组 - 未进行脊髓损伤的椎板切除术。GSH和SS通过腹腔注射给药。第1组和第4组未接受干预。
在脊髓损伤后的第2、7、14、21、28、35和42天，通过Basso、Beattie和Bresnahan（BBB）量表在七个不同时间点评估大鼠的运动功能恢复情况。在第42天，处死大鼠以分析损伤脊髓的组织学结果。在接受GSH的组中，我们的实验研究显示在BBB量表、水平阶梯量表以及头侧和尾侧轴突计数方面有更好的功能评分。在BBB评分和轴突计数分析中发现的差异具有统计学意义。
本研究表明，在实验性脊髓创伤中使用谷胱甘肽可使接受实验性脊髓损伤的Wistar大鼠实现更好的功能恢复并提高轴突再生率。
