高压氧疗法增强 GM1 在急性实验性大鼠脊髓损伤中的作用。

Potentializing the effects of GM1 by hyperbaric oxygen therapy in acute experimental spinal cord lesion in rats.

机构信息

Department of Orthopaedics and Traumatology, University São Paulo Medicine School, São Paulo, Brazil.

出版信息

Spinal Cord. 2010 Nov;48(11):808-13. doi: 10.1038/sc.2010.37. Epub 2010 Apr 27.

DOI:10.1038/sc.2010.37

PMID:20421874

Abstract

STUDY DESIGN

Experimental, controlled, animal study.

OBJECTIVES

To evaluate the effect of GM1 ganglioside, hyperbaric oxygen and both in combination, in the treatment of experimental spinal cord lesions in rats.

SETTING

Brazil.

METHODS

Thirty-two Wistar rats with spinal cord lesions were divided into four groups: one group received GM1 ganglioside, one was submitted to hyperbaric oxygen therapy (HBOT), the third received both treatments and the fourth received no treatment (control).

RESULTS

There were no significant differences between the groups in the histological analysis, for any of the variables (necrosis, hemorrhage, hyperemia, cystic degeneration, P>0.06). Neither were there any significant differences in the comparison of left and right sides in the functional tests (P>0.06 for all). No significant differences were found in the locomotor ratings, in the comparison of groups at 2, 7, 21 and 28 days after the surgical procedure. However, in the evaluation on day 14, group 3, which received the combined therapy, showed a significantly higher Basso Beattie and Bresnahan score than the other groups (P=0.015).

CONCLUSION

The therapeutic effect of GM1 in locomotor evaluation of rats submitted to spinal cord lesion is anticipated by HBOT.

摘要

研究设计

实验性、对照、动物研究。

目的

评估 GM1 神经节苷脂、高压氧及两者联合治疗大鼠实验性脊髓损伤的效果。

地点

巴西。

方法

32 只脊髓损伤大鼠分为四组：一组给予 GM1 神经节苷脂，一组接受高压氧治疗（HBOT），第三组接受两种治疗，第四组不接受治疗（对照组）。

结果

在组织学分析中，各组间（坏死、出血、充血、囊性变性）的任何变量均无显著差异（P>0.06）。在功能测试中，左右侧比较也无显著差异（所有 P>0.06）。在手术 2、7、21 和 28 天后的组间比较中，运动评分无显著差异。然而，在第 14 天的评估中，接受联合治疗的第 3 组的 Basso Beattie 和 Bresnahan 评分明显高于其他组（P=0.015）。