GIGA Neurosciences, University of Liege, Liege, Belgium.
J Neurochem. 2010 Feb;112(3):762-72. doi: 10.1111/j.1471-4159.2009.06500.x. Epub 2009 Nov 18.
Ependymal cells located around the central canal of the adult spinal cord are considered as a source of neural stem cells (NSCs) and represent an interesting pool of endogenous stem cells for repair strategies. Physical exercise is known to increase ependymal cell proliferation, while improving functional recovery. In this work, we further characterized those endogenous NSCs within the normal and injured adult rat spinal cord and investigated the effects of treadmill training using immunohistochemical and behavioral studies. In uninjured untrained rats, Sox-2, a NSC marker, was detected in all ependymal cells of the central canal, and also scattered throughout the parenchyma of the spinal cord. Within the lesion, Sox-2 expression increased transiently, while the number of nestin-positive ependymal cells increased with a concomitant enhancement of proliferation, as indicated by the mitotic markers Ki67 and bromo-deoxyuridine. Exercise, which improved functional recovery and autonomous micturition, maintained nestin expression in both injured and uninjured spinal cords, with a positive correlation between locomotor recovery and the number of nestin-positive cells.
位于成人脊髓中央管周围的室管膜细胞被认为是神经干细胞（NSCs）的来源，代表了修复策略中内源性干细胞的一个有趣来源。运动已知可以增加室管膜细胞的增殖，同时改善功能恢复。在这项工作中，我们进一步描述了正常和受伤的成年大鼠脊髓内的这些内源性 NSCs，并通过免疫组织化学和行为研究调查了跑步机训练的影响。在未经训练的未受伤大鼠中，NSC 标志物 Sox-2 检测到中央管中所有室管膜细胞的存在，并且也散布在脊髓实质中。在损伤部位，Sox-2 的表达短暂增加，而巢蛋白阳性室管膜细胞的数量增加，同时增殖增强，有丝分裂标志物 Ki67 和溴脱氧尿苷也表明了这一点。运动改善了功能恢复和自主排尿，在受伤和未受伤的脊髓中均维持了巢蛋白的表达，运动恢复与巢蛋白阳性细胞数量之间存在正相关。
