Department of Nursing I, University of the Basque Country UPV/EHU, Spain; Department of Immunology, Microbiology and Parasitology, University of the Basque Country UPV/EHU, Spain.
Department of Immunology, Microbiology and Parasitology, University of the Basque Country UPV/EHU, Spain.
Diagn Microbiol Infect Dis. 2024 Jul;109(3):116311. doi: 10.1016/j.diagmicrobio.2024.116311. Epub 2024 Apr 18.
The detection of patterns associated with the invasive form of Candida albicans, such as Candida albicans germ tube antibodies (CAGTA), is a useful complement to blood culture for Invasive Candidiasis (IC) diagnosis. As CAGTA are detected by a non-standardisable and non-automatable technique, a Candida albicans cDNA expression library was screened with CAGTA isolated from serum of an animal model of invasive candidiasis, and five protein targets were identified: hyphally regulated cell wall protein 1 (Hyr1), enolase 1 (Eno1), coatomer subunit gamma (Sec21), a metallo-aminopeptidase (Ape2) and cystathionine gamma-lyase (Cys3). Homology with proteins from other organisms rules out Cys3 as a good biomarker while Sec21 results suggest that it is not in the germ tubes surface but secreted to the external environment. Our analysis propose Ape2, Sec21 and a region of Hyr1 different from the one currently being studied for immunoprotection as potential biomarker candidates for the diagnosis of IC.
检测与侵袭性白色念珠菌（如白色念珠菌芽管抗体（CAGTA））相关的模式是侵袭性念珠菌病（IC）诊断中血液培养的有益补充。由于 CAGTA 是通过非标准化和非自动化技术检测的，因此使用从侵袭性念珠菌病动物模型的血清中分离的 CAGTA 筛选了白色念珠菌 cDNA 表达文库，并鉴定了五个蛋白质靶标：菌丝调节细胞壁蛋白 1（Hyr1）、烯醇酶 1（Eno1）、衣壳亚基γ（Sec21）、金属氨基肽酶（Ape2）和半胱氨酸γ-裂解酶（Cys3）。与其他生物体的蛋白质的同源性排除了 Cys3 作为良好的生物标志物的可能性，而 Sec21 的结果表明它不在芽管表面，而是分泌到外部环境中。我们的分析提出 Ape2、Sec21 和 Hyr1 的一个不同于当前正在研究免疫保护的区域作为 IC 诊断的潜在生物标志物候选物。
