Hashimi Said M, Huang Minjun, Chen Yu, Liu Jianzhong
College of Life Sciences, Zhejiang Normal University, Jinhua 321004, Zhejiang, China.
Institute of Genetics and Developmental Biology, College of Life Sciences, Zhejiang Normal University, Jinhua 321004, Zhejiang, China.
Sheng Wu Gong Cheng Xue Bao. 2024 Apr 25;40(4):1065-1075. doi: 10.13345/j.cjb.230452.
Autophagy plays an essential role in recycling/re-utilizing nutrients and in adaptions to numerous stresses. However, the roles of autophagy in soybean have not been investigated extensively. In this study, a virus-induced gene silencing approach mediated by bean pod mottle virus (BPMV) was used to silence () genes in soybean (referred to as ). Our results showed that ATG8 proteins were massively accumulated in the dark-treated leaves of the -silenced plants relative to the vector control plants (BPMV-0), indicating that autophagy pathway is impaired in the -silenced plants. Consistent with the impaired autophagy, an accelerated senescence phenotype was observed on the leaves of the dark-treated -silenced plants, which was not shown on the leaves of the dark-treated BPMV-0 plants. In addition, the accumulation levels of both reactive oxygen species (ROS) and salicylic acid (SA) were significantly induced in the -silenced plants compared with that of the vector control plants (BPMV-0), indicating an activated immunity. Accordingly, the -silenced plants exhibited significantly enhanced resistance against pv. () in comparison with the BPMV-0 plants. Nevertheless, the activated immunity observed in the -silenced plant was independent of the activation of mitogen-activated protein kinase (MAPK).
自噬在营养物质的循环利用以及对多种胁迫的适应过程中发挥着至关重要的作用。然而，自噬在大豆中的作用尚未得到广泛研究。在本研究中，采用由菜豆荚斑驳病毒（BPMV）介导的病毒诱导基因沉默方法，在大豆中沉默（）基因（称为）。我们的结果表明，相对于载体对照植株（BPMV-0），在经黑暗处理的-沉默植株的叶片中，ATG8蛋白大量积累，这表明-沉默植株中的自噬途径受损。与自噬受损相一致，在经黑暗处理的-沉默植株的叶片上观察到加速衰老的表型，而在经黑暗处理的BPMV-0植株的叶片上未出现这种表型。此外，与载体对照植株（BPMV-0）相比，-沉默植株中活性氧（ROS）和水杨酸（SA）的积累水平均显著升高，表明免疫被激活。因此，与BPMV-0植株相比，-沉默植株对（）表现出显著增强的抗性。然而，在-沉默植株中观察到的免疫激活与丝裂原活化蛋白激酶（MAPK）的激活无关。
