Institute of Plant Genetics and Developmental Biology, College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China.
Zhejiang Provincial Key Laboratory of Biotechnology on Specialty Economic Plants,Zhejiang Normal University, Jinhua 321004, China.
Int J Mol Sci. 2021 Oct 29;22(21):11749. doi: 10.3390/ijms222111749.
Autophagy plays a critical role in nutrient recycling and stress adaptations. However, the role of autophagy has not been extensively investigated in crop plants. In this study, soybean () was silenced, using virus-induced silencing (VIGS) mediated by (BPMV). An accelerated senescence phenotype was exclusively observed for the silenced plants under dark conditions. In addition, significantly increased accumulation of both ROS and SA as well as a significantly induced expression of the pathogenesis-related gene 1 () were also observed on the leaves of the silenced plants, indicating an activated immune response. Consistent with this, -silenced plants exhibited a significantly enhanced resistance to pv. (Psg) relative to empty vector control plants (BPMV-0). Notably, the activated immunity of the silenced plants was independent of the MAPK signaling pathway. The fact that the accumulation levels of ATG8 protein and poly-ubiquitinated proteins were significantly increased in the dark-treated silenced plants relative to the BPMV-0 plants indicated that the autophagic degradation is compromised in the silenced plants. Together, our results indicated that silencing compromises the autophagy pathway, and the autophagy pathway is conserved in different plant species.
自噬在营养物质循环和应激适应中起着关键作用。然而，自噬在作物中的作用尚未得到广泛研究。在这项研究中，使用 （BPMV）介导的病毒诱导的沉默（VIGS）沉默大豆（）。在黑暗条件下，沉默植株表现出明显的加速衰老表型。此外，还观察到沉默植株叶片中 ROS 和 SA 的积累显著增加，以及病程相关基因 1（）的表达显著诱导，表明激活了免疫反应。与此一致，与空载体对照植物（BPMV-0）相比，沉默 pv. （Psg）的植物表现出显著增强的抗性（）。值得注意的是，沉默植株的激活免疫反应独立于 MAPK 信号通路。事实上，与 BPMV-0 植物相比，黑暗处理的沉默植物中 ATG8 蛋白和多聚泛素化蛋白的积累水平显著增加，表明沉默植物中的自噬降解受到损害。总之，我们的结果表明，沉默会损害自噬途径，而自噬途径在不同的植物物种中是保守的。
