Song Yongsen, Yu Yang, Tan Chuhe, Meng Qi, Gou Yurui, Duan Xiangbo
College of Life Science and Engineering, Shenyang University, Shenyang 110044, Liaoning, China.
Liaoning Key Laboratory of Urban Integrated Pest Management and Ecological Security, Shenyang 110044, Liaoning, China.
Sheng Wu Gong Cheng Xue Bao. 2024 Apr 25;40(4):1157-1169. doi: 10.13345/j.cjb.230681.
Cytokinin response factors (CRFs), as unique transcription factors in plants, play crucial roles in regulating development, phytohormone signaling pathway, and stress responses. In this study, we identified nine genes from the rice genome by conducting a BLAST analysis using the protein sequences of twelve AtCRFs. These genes are located on seven different rice chromosomes. We conducted a comprehensive analysis of the conserved domains, physicochemical properties, secondary structures, and phylogenetic relationships of rice CRF proteins using various online tools and local software. Additionally, we analyzed the exon-intron structures and -acting elements of , and found an abundance of elements relevant to phytohormone response and stress response on the promoters of rice genes. Spatial-temporal expression pattern analysis revealed that four of the were barely expressed in all tested samples, while the other five were highly expressed in the leaf, panicle, or seed of rice. Microarray data showed that genes are regulated to varying degrees by abscisic acid, auxin, cytokinin, and jasmonic acid. Furthermore, through analyzing the RNA-seq data, we found that are primarily involved in plant response to temperature stress (chilling and heat), with several also implicated in drought response, while hardly any respond to salt stress. This study provides an important basis for the functional characterization of rice family genes.
细胞分裂素响应因子（CRFs）作为植物中独特的转录因子，在调节植物发育、植物激素信号通路和应激反应中发挥着关键作用。在本研究中，我们通过使用12个拟南芥CRF蛋白序列进行BLAST分析，从水稻基因组中鉴定出9个基因。这些基因位于7条不同的水稻染色体上。我们使用各种在线工具和本地软件对水稻CRF蛋白的保守结构域、理化性质、二级结构和系统发育关系进行了全面分析。此外，我们分析了水稻CRF基因的外显子-内含子结构和顺式作用元件，发现在水稻CRF基因的启动子上有大量与植物激素反应和应激反应相关的元件。时空表达模式分析表明，9个基因中有4个在所有测试样品中几乎不表达，而其他5个在水稻的叶、穗或种子中高表达。微阵列数据表明，水稻CRF基因受到脱落酸、生长素、细胞分裂素和茉莉酸的不同程度调控。此外，通过分析RNA-seq数据，我们发现水稻CRF基因主要参与植物对温度胁迫（冷害和热害）的反应，有几个基因也参与干旱反应，而几乎没有基因对盐胁迫作出反应。本研究为水稻CRF家族基因的功能鉴定提供了重要依据。
