Bagde Nilajkumar D, Bagde Madhuri N, Agrawal Sarita, Nayak Prasanta, Negi Sanjay Singh, Rajbhar Sarita, Hussain Nighat
Obstetrics and Gynaecology, All India Institute of Medical Sciences Raipur, Raipur, IND.
Obstetrics and Gynaecology, Raipur Institute of Medical Sciences, Raipur, IND.
Cureus. 2024 Mar 25;16(3):e56864. doi: 10.7759/cureus.56864. eCollection 2024 Mar.
To determine the role of geminin as a tool for differentiating various types of cervical intraepithelial neoplasia (CIN) and cervical carcinoma (CC).
Seventy women newly diagnosed with CIN or CC undergoing cervical biopsy were included; their clinical profile, human papilloma virus (HPV) positivity, and colposcopy findings were noted, and biopsy tissue was analyzed for geminin content.
On geminin immunohistochemistry, 100% of women with CIN3 and 96.29% of women with CC had geminin two plus or more. When analyzed as ordinal variables, there was a significant correlation (spearman's rho 0.35, p 0.01) between geminin and biopsy results (CIN1, CIN2, CIN3, and CC).
Screening tests for cervical cancer, like conventional pap smears, liquid-based pap smears, and triaging with HPV, have limitations. It is important to be able to differentiate between high-grade lesions, invasive cancer, and low-grade lesions. The detection of geminin in these cells may aid in the confirmation of the diagnosis and ensure adequate treatment. Cervical intraepithelial lesions and carcinoma cervix demonstrated a correlation between increased geminin expression in CIN1 vs. CC and CIN2 vs. CC. Geminin may be a potential surrogate marker for higher-grade cervical lesions, and further research is needed to corroborate evidence in this direction.
确定geminin作为鉴别不同类型宫颈上皮内瘤变（CIN）和宫颈癌（CC）的工具的作用。
纳入70例新诊断为CIN或CC并接受宫颈活检的女性；记录她们的临床资料、人乳头瘤病毒（HPV）阳性情况和阴道镜检查结果，并分析活检组织中的geminin含量。
在geminin免疫组化中，100%的CIN3女性和96.29%的CC女性geminin为2+或更高。当作为有序变量分析时，geminin与活检结果（CIN1、CIN2、CIN3和CC）之间存在显著相关性（斯皮尔曼等级相关系数0.35，p<0.01）。
宫颈癌筛查试验，如传统巴氏涂片、液基巴氏涂片和HPV分流，存在局限性。能够区分高级别病变、浸润癌和低级别病变很重要。在这些细胞中检测geminin可能有助于确诊并确保充分治疗。宫颈上皮内病变和宫颈癌在CIN1与CC以及CIN2与CC之间显示出geminin表达增加的相关性。Geminin可能是高级别宫颈病变的潜在替代标志物，需要进一步研究来证实这方面的证据。
