Xing Yan, Wang Chaojun, Wu Jie
State Key Laboratory of Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing, China.
Medicine (Baltimore). 2017 Jun;96(26):e7302. doi: 10.1097/MD.0000000000007302.
Geminin is a protein involved in cell cycle progression. We aimed to evaluate the diagnostic value of geminin expression in cervical intraepithelial neoplasm (CIN).The expression of geminin, p16, and Ki67 was examined in 95 samples, including CIN1 (n = 45), CIN2/3 (n = 40), and normal cervical tissues (n = 10) by immunohistochemistry. The correlation between geminin or p16 expression and human papillomavirus (HPV) status was also evaluated.Geminin expression was negative in all normal tissues and expressed in 13.3% of CIN1 and 90.0% of CIN2/3. P16 expression was demonstrated in 24.4% of CIN1 and 87.5% of CIN2/3. The corresponding Ki67 expression was 35.6% and 95.0%. The specificity of geminin for differentiating between CIN1 and CIN2/3 was 86.7%, while for p16 and Ki67 the corresponding values were 75.6% and 64.4%. The sensitivity of geminin, p16, and Ki67 was 90.0%, 87.5%, and 95.0%, respectively. The positive predictive value (PPV) and accuracy of geminin were higher than p16 and Ki67. In addition, geminin expression showed a weak correlation with HPV status, but there was no association between p16 expression and HPV status.These results suggested that geminin had a high degree of sensitivity and specificity in determining CIN2/3. In addition to p16 and Ki67, geminin might be used as a new biomarker to distinguish between CIN1 and CIN2/3.
Geminin是一种参与细胞周期进程的蛋白质。我们旨在评估Geminin表达在宫颈上皮内瘤变（CIN）中的诊断价值。通过免疫组织化学检测了95个样本中Geminin、p16和Ki67的表达，这些样本包括CIN1（n = 45）、CIN2/3（n = 40）和正常宫颈组织（n = 10）。还评估了Geminin或p16表达与人类乳头瘤病毒（HPV）状态之间的相关性。Geminin在所有正常组织中均呈阴性表达，在13.3%的CIN1和90.0%的CIN2/3中表达。p16在24.4%的CIN1和87.5%的CIN2/3中表达。相应的Ki67表达分别为35.6%和95.0%。Geminin区分CIN1和CIN2/3的特异性为86.7%，而p16和Ki67的相应值分别为75.6%和64.4%。Geminin、p16和Ki67的敏感性分别为90.0%、87.5%和95.0%。Geminin的阳性预测值（PPV）和准确性高于p16和Ki67。此外，Geminin表达与HPV状态呈弱相关，但p16表达与HPV状态之间无关联。这些结果表明，Geminin在确定CIN2/3方面具有高度的敏感性和特异性。除了p16和Ki67外，Geminin可能作为区分CIN1和CIN2/3的新生物标志物。
