Kapoor Suman, Mihalovičová Lucia, Pisareva Ekaterina, Pastor Brice, Mirandola Alexia, Roch Benoit, Bryant Joe, Princy Anna Philip, Chouaib Salem, Thierry Alain Roger
Thumbay Research Institute for Precision Medicine, Gulf Medical University, Ajman, UAE.
IRCM, Institute of Research in Cancerology of Montpellier, INSERM U1194, Centre Hospitalier Universitaire, University of Montpellier, Montpellier, France.
iScience. 2024 Mar 26;27(5):109573. doi: 10.1016/j.isci.2024.109573. eCollection 2024 May 17.
We examined from a large exploratory study cohort of COVID-19 patients ( = 549) a validated panel of neutrophil extracellular traps (NETs) markers in different categories of disease severity. Neutrophil elastase (NE), myeloperoxidase (MPO), and circulating nuclear DNA (cir-nDNA) levels in plasma were seen to gradually and significantly ( < 0.0001) increase with the disease severity: mild (3.7, 48.9, and 15.8 ng/mL, respectively); moderate (9.8, 77.5, and 27.7 ng/mL, respectively); severe (11.7, 99.5, and 29.0 ng/mL, respectively); and critical (13.1, 110.2, and 46.0 ng/mL, respectively); and are also statistically different with healthy individuals (N = 140; < 0.0001). All observations made in relation to the Delta variant-infected patients are in line with Omicron-infected patients. We unexpectedly observed significantly higher levels of NETs in asymptomatic individuals as compared to healthy subjects ( < 0.0001). Moreover, the balance of cir-nDNA and circulating mitochondrial DNA level was affected in COVID-19 infected patients attesting to mitochondrial dysfunction.
我们从一个大型的COVID-19患者探索性研究队列（n = 549）中，检测了不同疾病严重程度类别下经过验证的中性粒细胞胞外诱捕网（NETs）标志物组。血浆中的中性粒细胞弹性蛋白酶（NE）、髓过氧化物酶（MPO）和循环核DNA（cir-nDNA）水平随着疾病严重程度逐渐且显著升高（P < 0.0001）：轻度（分别为3.7、48.9和15.8 ng/mL）；中度（分别为9.8、77.5和27.7 ng/mL）；重度（分别为11.7、99.5和29.0 ng/mL）；危重度（分别为13.1、110.2和46.0 ng/mL）；并且与健康个体（N = 140；P < 0.0001）相比也有统计学差异。所有关于感染德尔塔变异株患者的观察结果与感染奥密克戎变异株患者一致。我们意外地观察到，与健康受试者相比，无症状个体的NETs水平显著更高（P < 0.0001）。此外，COVID-19感染患者的cir-nDNA与循环线粒体DNA水平的平衡受到影响，证明存在线粒体功能障碍。
