血管活性肠肽与去甲肾上腺素协同作用，降低大脑皮层神经元的自发放电率。
Vasoactive intestinal polypeptide acts synergistically with norepinephrine to depress spontaneous discharge rate in cerebral cortical neurons.
作者信息
Ferron A, Siggins G R, Bloom F E
出版信息
Proc Natl Acad Sci U S A. 1985 Dec;82(24):8810-2. doi: 10.1073/pnas.82.24.8810.
Cortical neurons are densely innervated by noradrenergic fibers and by intrinsic cortical interneurons containing vasoactive intestinal polypeptide (VIP). Biochemically, VIP and norepinephrine (NE) synergistically interact to stimulate the synthesis of cyclic AMP in cortical slices. Therefore, we sought physiological indices of this peptide-monoamine interaction by applying VIP and NE to single cortical neurons of the rat while recording their spontaneous discharge. VIP applied alone inhibited discharge of 24% and accelerated discharge in 20% of cortical neurons. NE alone had a predominantly depressant effect on the same neurons. However, when VIP was retested during the continuous application of subthreshold currents of NE, VIP exerted predominantly depressant effects. These synergistic inhibitions resulted even in cells previously showing excitations to VIP alone. If VIP alone was depressant, subthreshold NE further enhanced the VIP depression. Subthreshold amounts of phenylephrine, an alpha-adrenoceptor agonist, also produced comparable interactions, suggesting involvement of an alpha receptor, as in the biochemical studies. These results support a peptide-monoamine interaction in cortex that could have important ramifications for neuronal integration.
皮质神经元由去甲肾上腺素能纤维以及含有血管活性肠肽（VIP）的皮质内源性中间神经元密集支配。在生化方面，VIP和去甲肾上腺素（NE）协同相互作用，刺激皮质切片中环磷酸腺苷（cAMP）的合成。因此，我们通过将VIP和NE应用于大鼠单个皮质神经元并记录其自发放电，来寻找这种肽 - 单胺相互作用的生理指标。单独应用VIP时，24%的皮质神经元放电受到抑制，20%的皮质神经元放电加速。单独使用NE对相同神经元主要产生抑制作用。然而，当在持续施加阈下电流的NE期间再次测试VIP时，VIP主要发挥抑制作用。这些协同抑制作用甚至在先前单独对VIP表现出兴奋的细胞中也会出现。如果单独使用VIP具有抑制作用，阈下剂量的NE会进一步增强VIP的抑制作用。阈下剂量的α-肾上腺素能受体激动剂去氧肾上腺素也会产生类似的相互作用，这表明如同生化研究中一样，α受体参与其中。这些结果支持皮质中存在肽 - 单胺相互作用，这可能对神经元整合具有重要影响。
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