Jilin Provincial Key Laboratory of Nutrition and Functional Food and College of Food Science and Engineering, Jilin University, Changchun 130062, People's Republic of China.
Jilin Provincial Key Laboratory of Nutrition and Functional Food and College of Food Science and Engineering, Jilin University, Changchun 130062, People's Republic of China.
Food Chem. 2024 Sep 1;451:139337. doi: 10.1016/j.foodchem.2024.139337. Epub 2024 Apr 15.
Alcoholic liver disease (ALD) is a serious health threat. Soybean meal peptide (SMP) supplementation may protect against this damage; however, the potential mechanism underlying the specific sequence of SMPs is unclear. Protein-protein interaction and proteomic analyses are effective methods for studying functional ingredients in diseases. This study aimed to investigate the potential mechanism of action of the peptide Gly-Thr-Tyr-Trp (GTYW) on ALD using protein-protein interaction and proteomic analyses. These results demonstrate that GTYW influenced the targets of glutathione metabolism (glutathione-disulfide reductase, glutathione S-transferase pi 1, and glutathione S-transferase mu 2). It also regulated the expression of targets related to energy metabolism and amino acid conversion (trypsin-2, cysteine dioxygenase type-1, and F6SJM7). Amino acid and lipid metabolisms were identified based on Gene Ontology annotation. These results indicate that GTYW might affect alcohol-related liver disease signaling pathways. This study provides evidence of the protective and nutritional benefits of SMPs in ALD treatment.
酒精性肝病（ALD）是一种严重的健康威胁。大豆肽（SMP）补充可能有助于预防这种损害；然而，SMP 特定序列的潜在机制尚不清楚。蛋白质-蛋白质相互作用和蛋白质组学分析是研究疾病中功能性成分的有效方法。本研究旨在使用蛋白质-蛋白质相互作用和蛋白质组学分析研究肽 Gly-Thr-Tyr-Trp（GTYW）对 ALD 的潜在作用机制。这些结果表明，GTYW 影响了谷胱甘肽代谢的靶点（谷胱甘肽二硫还原酶、谷胱甘肽 S-转移酶 pi 1 和谷胱甘肽 S-转移酶 mu 2）。它还调节与能量代谢和氨基酸转化相关的靶标（胰蛋白酶-2、半胱氨酸双加氧酶 1 型和 F6SJM7）的表达。基于基因本体注释鉴定了氨基酸和脂质代谢。这些结果表明，GTYW 可能影响与酒精相关的肝病信号通路。本研究为 SMP 在 ALD 治疗中的保护和营养益处提供了证据。
