Chen Bingzhi, Chen Juanqin, Wu Huihua, Zhang Fangyi, Chen Lili, Zhang Weibin, Yang Jing, Yuan Li, Jiang Yuji, Deng Youjin
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Foods. 2025 Apr 29;14(9):1557. doi: 10.3390/foods14091557.
This study investigated the hepatoprotective mechanisms of fruiting body polypeptide (VVFP, 1-3 kDa) against acute alcohol-induced liver injury using multi-omics approaches. Male ICR mice pretreated with VVFP (100-400 mg/kg) showed significantly prolonged alcohol tolerance latency ( < 0.05) and accelerated sobriety recovery compared to controls. Integrated transcriptomics and metabolomics revealed VVFP's dual regulatory effects: (1) transcriptional regulation of 36 endoplasmic reticulum stress genes (e.g., , ) through protein processing pathways (KEGG:04141), and (2) metabolic modulation of 23 hepatic metabolites, particularly phosphatidylcholines and organic acids, via amino acid biosynthesis and glycerophospholipid metabolism. Cross-omics analysis identified eight coregulated genes (, , , etc.) interacting with key metabolites (4-guanidinobutyric acid, GABA) through linoleic acid metabolism. These findings demonstrate VVFP's therapeutic potential as a functional food ingredient by highlighting its ability to simultaneously target hepatic stress responses and metabolic homeostasis during alcohol detoxification.
本研究采用多组学方法，探究了子实体多肽（VVFP，1 - 3 kDa）对急性酒精性肝损伤的肝保护机制。与对照组相比，用VVFP（100 - 400 mg/kg）预处理的雄性ICR小鼠酒精耐受潜伏期显著延长（<0.05），清醒恢复加快。综合转录组学和代谢组学揭示了VVFP的双重调节作用：（1）通过蛋白质加工途径（KEGG:04141）对36个内质网应激基因（如……）进行转录调控，以及（2）通过氨基酸生物合成和甘油磷脂代谢对23种肝脏代谢物，特别是磷脂酰胆碱和有机酸进行代谢调节。跨组学分析确定了八个共调控基因（……等）通过亚油酸代谢与关键代谢物（4 - 胍基丁酸，GABA）相互作用。这些发现突出了VVFP在酒精解毒过程中同时靶向肝脏应激反应和代谢稳态的能力，证明了其作为功能性食品成分的治疗潜力。
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