Holmberg Sandra M, Feeney Rachel H, Prasoodanan P K Vishnu, Puértolas-Balint Fabiola, Singh Dhirendra K, Wongkuna Supapit, Zandbergen Lotte, Hauner Hans, Brandl Beate, Nieminen Anni I, Skurk Thomas, Schroeder Bjoern O
Department of Molecular Biology, Umeå University, Umeå, Sweden.
Laboratory for Molecular Infection Medicine Sweden (MIMS) and Umeå Center for Microbial Research (UCMR), Umeå University, Umeå, Sweden.
Nat Commun. 2024 Apr 25;15(1):3502. doi: 10.1038/s41467-024-47594-w.
Beneficial gut bacteria are indispensable for developing colonic mucus and fully establishing its protective function against intestinal microorganisms. Low-fiber diet consumption alters the gut bacterial configuration and disturbs this microbe-mucus interaction, but the specific bacteria and microbial metabolites responsible for maintaining mucus function remain poorly understood. By using human-to-mouse microbiota transplantation and ex vivo analysis of colonic mucus function, we here show as a proof-of-concept that individuals who increase their daily dietary fiber intake can improve the capacity of their gut microbiota to prevent diet-mediated mucus defects. Mucus growth, a critical feature of intact colonic mucus, correlated with the abundance of the gut commensal Blautia, and supplementation of Blautia coccoides to mice confirmed its mucus-stimulating capacity. Mechanistically, B. coccoides stimulated mucus growth through the production of the short-chain fatty acids propionate and acetate via activation of the short-chain fatty acid receptor Ffar2, which could serve as a new target to restore mucus growth during mucus-associated lifestyle diseases.
有益的肠道细菌对于结肠黏液的形成以及充分建立其对肠道微生物的保护功能不可或缺。低纤维饮食会改变肠道细菌结构，扰乱这种微生物与黏液的相互作用，但对于维持黏液功能的特定细菌和微生物代谢产物仍知之甚少。通过使用人源化小鼠微生物群移植和结肠黏液功能的体外分析，我们在此作为概念验证表明，增加每日膳食纤维摄入量的个体可以提高其肠道微生物群预防饮食介导的黏液缺陷的能力。黏液生长是完整结肠黏液的一个关键特征，与肠道共生菌布劳特氏菌的丰度相关，向小鼠补充球形布劳特氏菌证实了其刺激黏液生长的能力。从机制上讲，球形布劳特氏菌通过激活短链脂肪酸受体Ffar2产生短链脂肪酸丙酸酯和乙酸酯来刺激黏液生长，这可以作为在黏液相关的生活方式疾病中恢复黏液生长的新靶点。
