Bhattacharjee Disha, Millman Lindsey C, Seesengood Meagan L, Seekatz Anna M
Department of Biological Sciences, Clemson University, Clemson, South Carolina, USA.
bioRxiv. 2025 May 1:2025.05.01.651700. doi: 10.1101/2025.05.01.651700.
Members of the class Clostridia, a polyphyletic group of Gram-positive, spore-forming anaerobes in the Bacillota (Firmicutes) phylum, are prevalent and commonly associated with beneficial functions, such as providing colonization resistance against pathogens. Despite a demonstrated value in maintaining Clostridial populations in the gut, the functional and strain diversity of most commensal Clostridial species remains understudied. Here, we isolated and characterized Clostridial isolates, focusing on the genomic diversity of , a prevalent butyrate-producing genus within the gut microbiota. We conducted a genomic comparison across 21 strains isolated from healthy human fecal samples (n = 5) and publicly available genomes (n = 105). Whole genome comparisons across the genus demonstrated 12 species bins, clustering into three major functionally distinct clusters correlating with host origin. One cluster (representing mostly genomes) was distinguished by possessing a complete vitamin B12 biosynthesis pathway. Variability in carbohydrate and amino acid metabolism was demonstrated within dominant species of the human microbiota (, and ). Collectively, these data indicate metabolic variance across species that may influence coexistence within the gut environment and variably influence health.
梭菌属（Clostridia）的成员是芽孢杆菌门（厚壁菌门）中革兰氏阳性、产芽孢厌氧菌的一个多系群，普遍存在且通常与有益功能相关，比如提供对病原体的定植抗性。尽管在维持肠道中梭菌种群方面已证明其价值，但大多数共生梭菌物种的功能和菌株多样性仍未得到充分研究。在此，我们分离并鉴定了梭菌分离株，重点关注肠道微生物群中一个普遍存在的产丁酸菌属的基因组多样性。我们对从健康人类粪便样本中分离出的21株菌株（n = 5）和公开可用的基因组（n = 105）进行了基因组比较。对整个属的全基因组比较显示有12个物种分类单元，聚集成三个主要的功能不同的簇，与宿主来源相关。一个簇（主要代表基因组）的特征是拥有完整的维生素B12生物合成途径。在人类微生物群的优势物种（、和）中显示出碳水化合物和氨基酸代谢的变异性。总体而言，这些数据表明不同物种间的代谢差异可能会影响肠道环境中的共存，并对健康产生不同影响。
