Gordon T P, Kowanko I C, James M, Roberts-Thomson P J
Clin Exp Rheumatol. 1985 Oct-Dec;3(4):291-6.
Monosodium urate crystal-induced inflammation was studied in an air pouch model of rat synovium. Phagocytosis of crystals by macrophage-like and fibroblast-like cells in the pouch lining was observed 30 minutes after crystal injection and preceded polymorphonuclear leukocyte accumulation. Levels of the prostaglandins PGE2 and 6-keto-PGF1 alpha, measured in serial pouch washouts, showed a clear temporal dissociation with leucocyte numbers, with peak levels of the prostanoids occurring before the maximum accumulation of leucocytes. Indomethacin abrogated prostaglandin synthesis completely without reducing leucocyte counts in pouch washouts. We propose that the pouch lining cells may be the primary source of the prostaglandins and these mediators do not have a significant role in leucocyte chemotaxis in this model.
在大鼠滑膜气囊模型中研究了尿酸单钠晶体诱导的炎症。晶体注射30分钟后，观察到气囊内衬中巨噬细胞样和成纤维细胞样细胞对晶体的吞噬作用，且早于多形核白细胞的聚集。在连续的气囊冲洗液中测量的前列腺素PGE2和6-酮-PGF1α水平与白细胞数量呈现明显的时间分离，前列腺素类物质的峰值水平出现在白细胞最大聚集之前。吲哚美辛完全消除了前列腺素的合成，但并未降低气囊冲洗液中的白细胞计数。我们认为，气囊内衬细胞可能是前列腺素的主要来源，并且这些介质在该模型的白细胞趋化作用中没有显著作用。
