i3N, Department of Physics, University of Aveiro, 3810-193 Aveiro, Portugal.
CICECO-Aveiro Institute of Materials, Department of Materials and Ceramics Engineering, University of Aveiro, 3810-193 Aveiro, Portugal.
Biosensors (Basel). 2024 Apr 4;14(4):173. doi: 10.3390/bios14040173.
Heart failure (HF) is a clinical entity included in cardiovascular diseases affecting millions of people worldwide, being a leading cause of hospitalization of older adults, and therefore imposing a substantial economic burden on healthcare systems. HF is characterized by dyspnea, fatigue, and edema associated with elevated blood levels of natriuretic peptides, such as N Terminal pro-B-type Natriuretic Peptide (NT-proBNP), for which there is a high demand for point of care testing (POCT) devices. Optical fiber (OF) biosensors offer a promising solution, capable of real-time detection, quantification, and monitoring of NT-proBNP concentrations in serum, saliva, or urine. In this study, immunosensors based on plasmonic uncladded OF tips were developed using OF with different core diameters (200 and 600 µm). The tips were characterized to bulk refractive index (RI), anddetection tests were conducted with NT-proBNP concentrations varying from 0.01 to 100 ng/mL. The 200 µm sensors showed an average total variation of 3.6 ± 2.5 mRIU, an average sensitivity of 50.5 mRIU/ng·mL, and a limit of detection (LOD) of 0.15 ng/mL, while the 600 µm sensors had a response of 6.1 ± 4.2 mRIU, a sensitivity of 102.8 mRIU/ng·mL, and an LOD of 0.11 ng/mL. Control tests were performed using interferents such as uric acid, glucose, and creatinine. The results show the potential of these sensors for their use in biological fluids.
心力衰竭（HF）是一种包含在影响全球数百万人的心血管疾病中的临床实体，是导致老年患者住院的主要原因，因此给医疗保健系统带来了巨大的经济负担。HF 的特征是呼吸困难、疲劳和水肿，同时血液中利钠肽等生物标志物水平升高，如 N 末端 pro-B 型利钠肽（NT-proBNP），这就需要床边即时检测（POCT）设备。光纤（OF）生物传感器提供了一个有前途的解决方案，能够实时检测、定量和监测血清、唾液或尿液中的 NT-proBNP 浓度。在这项研究中，使用具有不同芯径（200 和 600 µm）的 OF 开发了基于等离子体无涂层 OF 尖端的免疫传感器。对尖端进行了体折射率（RI）的特性表征，并进行了 NT-proBNP 浓度从 0.01 到 100 ng/mL 的检测测试。200 µm 传感器的平均总变化为 3.6 ± 2.5 mRIU，平均灵敏度为 50.5 mRIU/ng·mL，检测限（LOD）为 0.15 ng/mL，而 600 µm 传感器的响应为 6.1 ± 4.2 mRIU，灵敏度为 102.8 mRIU/ng·mL，检测限（LOD）为 0.11 ng/mL。使用尿酸、葡萄糖和肌酸酐等干扰物进行了对照测试。结果表明，这些传感器在生物流体中的应用具有潜力。
