Aydin Kurc Mine, Erfan Gamze, Kaya Ayse Demet, Gülen Dumrul, Oznur Meltem, Yanik Mehmet Emin
Department of Medical Microbiology, Faculty of Medicine, Tekirdag Namik Kemal University, Tekirdag 59030, Türkiye.
Department of Dermatology, Faculty of Medicine, Acıbadem University, Istanbul 34752, Türkiye.
Diagnostics (Basel). 2024 Apr 18;14(8):843. doi: 10.3390/diagnostics14080843.
Recent studies have focused on the role of human herpesvirus 6 (HHV-6) and human herpesvirus 7 (HHV-7) in PR etiology with varying results. In our study, with the approach that the discrepancy between the results may be related to the different samples and techniques used, we aimed to clarify the etiology by examining tissue and plasma samples using molecular methods and evaluating the results together with serological parameters. Skin biopsies and plasma samples of twenty-five PR patients were tested to detect HHV-6 and HHV-7 DNA using calibrated quantitative real-time polymerase chain reaction (CQ RT-PCR). IgG and IgM antibodies against HHV-6 and HHV-7 were tested by enzyme-linked immunosorbent assay and indirect immunofluorescence. Of the patient group, 64% were positive for HHV-6 IgG without IgM positivity. HHV-6 DNA was present in seven tissue and ten plasma samples. HHV-7 positivity was 100% and 12% for IgG and IgM antibodies, respectively. HHV-7 DNA was detected in four tissue samples and one plasma sample. Patients with HHV-7 DNA-positive plasma and tissue samples had also HHV-7 IgM antibodies. In conclusion, our results seem to support the role of HHV-6/HHV-7 in the etiology of PR. To clarify the etiology of PR and avoid confusion, the collection of different biological materials simultaneously and the usage of CQ RT-PCR as a diagnostic technique are recommended.
最近的研究聚焦于人类疱疹病毒6型（HHV-6）和人类疱疹病毒7型（HHV-7）在玫瑰糠疹（PR）病因学中的作用，结果各异。在我们的研究中，鉴于结果差异可能与所使用的不同样本和技术有关，我们旨在通过分子方法检测组织和血浆样本，并结合血清学参数评估结果来阐明病因。对25例PR患者的皮肤活检组织和血浆样本进行检测，使用校准定量实时聚合酶链反应（CQ RT-PCR）检测HHV-6和HHV-7 DNA。通过酶联免疫吸附测定和间接免疫荧光检测针对HHV-6和HHV-7的IgG和IgM抗体。在患者组中，64%的患者HHV-6 IgG呈阳性，而IgM无阳性。7份组织样本和10份血浆样本中存在HHV-6 DNA。HHV-7 IgG和IgM抗体的阳性率分别为100%和12%。在4份组织样本和1份血浆样本中检测到HHV-7 DNA。血浆和组织样本中HHV-7 DNA呈阳性的患者也有HHV-7 IgM抗体。总之，我们的结果似乎支持HHV-6/HHV-7在PR病因学中的作用。为了阐明PR的病因并避免混淆，建议同时收集不同生物材料并使用CQ RT-PCR作为诊断技术。
