Charoenchokpanich Wiriya, Muangrod Pratchaya, Roytrakul Sittiruk, Rungsardthong Vilai, Wonganu Benjamaporn, Charoenlappanit Sawanya, Casanova Federico, Thumthanaruk Benjawan
Department of Agro-Industrial, Food, and Environmental Technology, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok, Bangkok 10800, Thailand.
Functional Proteomics Technology Laboratory, National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand.
Gels. 2024 Apr 17;10(4):271. doi: 10.3390/gels10040271.
Due to its excellent biocompatibility and ease of biodegradation, jellyfish gelatin has gained attention as a hydrogel. However, hydrogel produced from jellyfish gelatin has not yet been sufficiently characterized. Therefore, this research aims to produce a jellyfish gelatin-based hydrogel. The gelatin produced from desalted jellyfish by-products varied with the part of the specimen and extraction time. Hydrogels with gelatin: glutaraldehyde ratios of 10:0.25, 10:0.50, and 10:1.00 (/) were characterized, and their cefazolin release ability was determined. The optimal conditions for gelatin extraction and chosen for the development of jellyfish hydrogels (JGel) included the use of the umbrella part of desalted jellyfish by-products extracted for 24 h (WU24), which yielded the highest gel strength (460.02 g), viscosity (24.45 cP), gelling temperature (12.70 °C), and melting temperature (22.48 °C). The quantities of collagen alpha-1(XXVIII) chain A, collagen alpha-1(XXI) chain, and collagen alpha-2(IX) chain in WU24 may influence its gel properties. Increasing the glutaraldehyde content in JGel increased the gel fraction by decreasing the space between the protein chains and gel swelling, as glutaraldehyde binds with lateral amino acid residues and produces a stronger network. At 8 h, more than 80% of the cefazolin in JGel (10:0.25) was released, which was higher than that released from bovine hydrogel (52.81%) and fish hydrogel (54.04%). This research is the first report focused on the production of JGel using glutaraldehyde as a cross-linking agent.
由于其优异的生物相容性和易于生物降解性，水母明胶作为一种水凝胶受到了关注。然而，由水母明胶制成的水凝胶尚未得到充分表征。因此，本研究旨在制备一种基于水母明胶的水凝胶。由脱盐水母副产物制成的明胶随标本部位和提取时间而变化。对明胶与戊二醛比例为10:0.25、10:0.50和10:1.00（/）的水凝胶进行了表征，并测定了它们的头孢唑啉释放能力。用于制备水母水凝胶（JGel）的明胶提取的最佳条件包括使用脱盐水母副产物的伞部提取24小时（WU24），其产生了最高的凝胶强度（460.02克）、粘度（24.45厘泊）、胶凝温度（12.70℃）和熔化温度（22.48℃）。WU24中胶原蛋白α-1(XXVIII)链A、胶原蛋白α-1(XXI)链和胶原蛋白α-2(IX)链的数量可能会影响其凝胶性能。增加JGel中戊二醛的含量会通过减少蛋白质链之间的空间和凝胶溶胀来增加凝胶分数，因为戊二醛与侧链氨基酸残基结合并产生更强的网络。在8小时时，JGel（10:0.25）中超过80%的头孢唑啉被释放，这高于从牛水凝胶（52.81%）和鱼水凝胶（54.04%）中释放的量。本研究是第一份专注于使用戊二醛作为交联剂生产JGel 的报告。
