Kalra S P, Kalra P S
Acta Endocrinol (Copenh). 1979 Sep;92(1):1-7. doi: 10.1530/acta.0.0920001.
Pro-oestrous-type LH surges were induced by treating ovariectomized rats (day 0) with either oestradiol benzoate (OeB, 40 micrograms/rat) or oestrogen (10 micrograms/rat followed by progesterone (4 mg/rat, day 2, OeBP). To characterize the temporal changes associated with the LH surge on day 2, LH-RH levels in the medial basal hypothalamus (MBH) and the pre-optic area (POA) were analyzed by RIA in rats sacrified at hourly intervals between 10.00--19.00 h. Following these treatments the LH-RH content of POA fluctuated somewhat but no definite pattern was observed. In OeB rats, the MBH LH-RH levels were unchanged through the initial LH surge period (13.00--15.00 h) declining significantly, as in the OeBP group, near the end of the surge period (19:00 h). However, in OeBP rats, the MBH LH-RH contents decreased at 14.00 h and these were followed by markedly elevated levels between 15.00--17.00 h coincident with maximal increments in serum LH. The OeBP group alone showed a surge of serum FSH.
通过给去卵巢大鼠（第0天）注射苯甲酸雌二醇（OeB，40微克/只）或雌激素（10微克/只，随后在第2天注射孕酮（4毫克/只，OeBP））来诱导发情前期类型的促黄体生成素（LH）高峰。为了描述与第2天LH高峰相关的时间变化，在10:00至19:00每小时处死一批大鼠，通过放射免疫分析法（RIA）分析内侧基底部下丘脑（MBH）和视前区（POA）中的促性腺激素释放激素（LH-RH）水平。经过这些处理后，POA中的LH-RH含量有所波动，但未观察到明确的模式。在OeB大鼠中，MBH的LH-RH水平在最初的LH高峰期间（13:00至15:00）保持不变，然后显著下降，与OeBP组一样，在高峰期末期（19:00）附近。然而，在OeBP大鼠中，MBH的LH-RH含量在14:00时下降，随后在15:00至17:00之间显著升高，与血清LH的最大增量同时出现。仅OeBP组出现了血清促卵泡生成素（FSH）高峰。
