Yanaihara T, Arai K, Kanazawa M, Okinaga S, Yanaihara N, Yanaihara C
Endocrinol Jpn. 1975 Dec;22(6):525-30. doi: 10.1507/endocrj1954.22.525.
A radioimmunoassay (RIA) method for luteinizing hormone (LH) releasing hormone (RH) utilizing rabbit antiserum against synthetic (Glu1)-LH-RH coupled with human serum albumin at the N-terminus, is described. This assay system for LH-RH also cross-reacted with several LH-RH analogues or fragments, but not with pituitary trophic hormones. The assay was performed on the hypothalamic extracts of adult ovariectomized rats and female immature rats which had been treated with estradiol. The FSH and LH levels in the pituitary gland and serum of the same animals were determined by RIA. The radioimmunoreactive LH-RH content of the stalk median eminence markedly increased seven days after ovariectomy. The serum levels and the pituitary contents of FSH and LH of the same rats were also significantly augmented. In immature rats, the hypothalamic content of LH-RH, as measured by RIA, was significantly increased one hour after the injection of estradiol. The FSH and LH levels in the pituitary showed a significant rise after 7 hours.
本文描述了一种利用兔抗血清检测促黄体生成素释放激素（LH-RH）的放射免疫分析法（RIA），该抗血清针对在N端与人血清白蛋白偶联的合成（Glu1）-LH-RH。这种LH-RH检测系统也与几种LH-RH类似物或片段发生交叉反应，但与垂体促性腺激素无交叉反应。该检测方法应用于成年去卵巢大鼠和经雌二醇处理的雌性幼鼠的下丘脑提取物。通过RIA测定相同动物垂体和血清中的FSH和LH水平。去卵巢7天后，垂体柄正中隆起的放射免疫反应性LH-RH含量显著增加。相同大鼠的血清FSH和LH水平以及垂体含量也显著升高。在幼鼠中，注射雌二醇1小时后，通过RIA测定的下丘脑LH-RH含量显著增加。垂体中的FSH和LH水平在7小时后显著升高。
