Yeh Chung-Hsin, Praveen Rajneesh Chellappan, Liao Chun-Hou, You Wen-Chen, Chen Kuo-Chiang, Wu Yi-No, Chiang Han-Sun
Division of Urology, Department of Surgery, Shin Kong Wu Ho-Su Memorial Hospital, Taipei City 111045, Taiwan.
School of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei City 242062, Taiwan.
Toxics. 2024 Mar 25;12(4):239. doi: 10.3390/toxics12040239.
This study explores the potential efficacy of chlorogenic acid (CGA) in mitigating lipopolysaccharide (LPS)-induced cystitis in a mice model. C57BL/6J mice were divided into four groups: normal control (NC), LPS, LPS + low CGA, and LPS + high CGA. Evaluation methods included cystometrogram (CMG), histopathological, western blot, and immunohistological analysis. In the LPS group, CMG revealed abnormal voiding behavior with increased micturition pressure, voided volume (VV), and decreased voided frequency. Low CGA treatment in LPS mice demonstrated improved micturition pressure and inter-contraction intervals (ICI). However, high CGA treatment exhibited prolonged ICI and increased VV, suggesting potential adverse effects. Histological analysis of LPS-treated mice displayed bladder inflammation and interstitial edema. Low CGA treatment reduced interstitial edema and bladder inflammation, confirmed by Masson's trichrome staining. Western blotting revealed increased cytokeratin 20 (K20) expression in the low CGA group, indicating structural abnormalities in the bladder umbrella layer after LPS administration. In conclusion, low CGA treatment positively impacted voiding behavior and decreased bladder edema and inflammation in the LPS-induced cystitis mice model, suggesting its potential as a supplement for inflammation cystitis prevention. However, high CGA treatment exhibited adverse effects, emphasizing the importance of dosage considerations in therapeutic applications.
本研究探讨了绿原酸（CGA）在减轻小鼠模型中脂多糖（LPS）诱导的膀胱炎方面的潜在疗效。将C57BL/6J小鼠分为四组：正常对照组（NC）、LPS组、LPS + 低剂量CGA组和LPS + 高剂量CGA组。评估方法包括膀胱压力容积测定法（CMG）、组织病理学、蛋白质免疫印迹法和免疫组织学分析。在LPS组中，CMG显示排尿行为异常，排尿压力、排尿量（VV）增加，排尿频率降低。LPS小鼠接受低剂量CGA治疗后，排尿压力和收缩间期（ICI）有所改善。然而，高剂量CGA治疗导致ICI延长和VV增加，提示可能存在不良反应。对LPS处理小鼠的组织学分析显示膀胱炎症和间质水肿。低剂量CGA治疗减轻了间质水肿和膀胱炎症，Masson三色染色证实了这一点。蛋白质免疫印迹法显示低剂量CGA组细胞角蛋白20（K20）表达增加，表明LPS给药后膀胱伞状层存在结构异常。总之，低剂量CGA治疗对LPS诱导的膀胱炎小鼠模型的排尿行为产生了积极影响，并减轻了膀胱水肿和炎症，表明其作为预防炎症性膀胱炎补充剂的潜力。然而，高剂量CGA治疗显示出不良反应，强调了在治疗应用中考虑剂量的重要性。
