Whitney Laboratory for Marine Biosciences University of Florida, St. Augustine, FL, USA.
Department of Neuroscience and McKnight Brain Institute, University of Florida, Gainesville, FL, USA.
Methods Mol Biol. 2024;2757:147-161. doi: 10.1007/978-1-0716-3642-8_5.
Ctenophores or comb jellies are representatives of an enigmatic lineage of early branching metazoans with complex tissue and organ organization. Their biology and even microanatomy are not well known for most of these fragile pelagic and deep-water species. Here, we present immunohistochemical protocols successfully tested on more than a dozen ctenophores. This chapter also illustrates neural organization in several reference species of the phylum (Pleurobrachia bachei, P. pileus, Mnemiopsis leidyi, Bolinopsis microptera, Beroe ovata, and B. abyssicola) as well as numerous ciliated structures in different functional systems. The applications of these protocols illuminate a very complex diversification of cell types comparable to many bilaterian lineages.
栉水母或栉水母是具有复杂组织和器官结构的早期分支后生动物的神秘谱系的代表。对于这些脆弱的浮游生物和深海物种中的大多数来说，它们的生物学甚至微观解剖学都知之甚少。在这里，我们提出了成功应用于十几种栉水母的免疫组织化学方案。本章还说明了门的几个参考物种（Pleurobrachia bachei、P. pileus、Mnemiopsis leidyi、Bolinopsis microptera、Beroe ovata 和 B. abyssicola）中的神经组织以及不同功能系统中的众多纤毛结构。这些方案的应用阐明了与许多两侧对称谱系相当的非常复杂的细胞类型多样化。
