Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria, College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China.
School of Urban and Environmental Sciences, Key Laboratory of the Ministry of Education for Earth Surface Processes, Peking University, Beijing 100871, China.
Environ Sci Technol. 2024 May 7;58(18):7758-7769. doi: 10.1021/acs.est.4c00122. Epub 2024 Apr 26.
Polycyclic aromatic hydrocarbon (PAH) exposure is suspected to be linked to oxidative damage. Herein, ten PAH human exposure biomarkers [hydroxylated PAH metabolites (OH-PAHs)] and five oxidative stress biomarkers (OSBs) were detected in urine samples collected from participants living in a rural area ( = 181) in Northwestern China. The median molar concentration of ΣOH-PAHs in urine was 47.0 pmol mL. The 2-hydroxynaphthalene (2-OHNap; median: 2.21 ng mL) was the dominant OH-PAH. The risk assessment of PAH exposure found that hazard index (HI) values were <1, indicating that the PAH exposure of rural people in Jingyuan would not generate significant cumulative risks. Smokers (median: 0.033) obtained higher HI values than nonsmokers (median: 0.015, < 0.01), suggesting that smokers face a higher health risk from PAH exposure than nonsmokers. Pearson correlation and multivariate linear regression analysis revealed that ΣOH-PAH concentrations were significant factors in increasing the oxidative damage to deoxyribonucleic acid (DNA) (8-hydroxy-2'-deoxyguanosine, 8-OHdG), ribonucleic acid (RNA) (8-oxo-7,8-dihydroguanine, 8-oxoGua), and protein (, '-dityrosine, diY) ( < 0.05). Among all PAH metabolites, only 1-hydroxypyrene (1-OHPyr) could positively affect the expression of all five OSBs ( < 0.05), suggesting that urinary 1-OHPyr might be a reliable biomarker for PAH exposure and a useful indicator for assessing the impacts of PAH exposure on oxidative stress. This study is focused on the relation between PAH exposure and oxidative damage and lays a foundation for the study of the health effect mechanism of PAHs.
多环芳烃 (PAH) 暴露被怀疑与氧化损伤有关。本研究检测了来自中国西北部农村地区（= 181）的参与者尿液中 10 种 PAH 人体暴露生物标志物[羟基化 PAH 代谢物 (OH-PAHs)]和 5 种氧化应激生物标志物 (OSBs)。尿液中ΣOH-PAHs 的中值摩尔浓度为 47.0 pmol mL。2-羟基萘（2-OHNap；中位数：2.21ng mL）是主要的 OH-PAH。PAH 暴露风险评估发现，危害指数 (HI) 值均<1，表明靖远农村人群的 PAH 暴露不会产生显著的累积风险。吸烟者（中位数：0.033）的 HI 值高于不吸烟者（中位数：0.015，<0.01），表明与不吸烟者相比，吸烟者因 PAH 暴露而面临更高的健康风险。Pearson 相关和多元线性回归分析表明，ΣOH-PAH 浓度是导致脱氧核糖核酸（DNA）（8-羟基-2'-脱氧鸟苷，8-OHdG）、核糖核酸（RNA）（8-氧代-7,8-二氢鸟嘌呤，8-oxoGua）和蛋白质（3,3'-二酪氨酸，diY）氧化损伤增加的重要因素（<0.05）。在所有 PAH 代谢物中，只有 1-羟基芘（1-OHPyr）能够正向影响所有 5 种 OSBs 的表达（<0.05），这表明尿 1-OHPyr 可能是 PAH 暴露的可靠生物标志物，也是评估 PAH 暴露对氧化应激影响的有用指标。本研究重点关注 PAH 暴露与氧化损伤之间的关系，为研究 PAHs 的健康效应机制奠定了基础。
