School of Materials Science and Engineering, University of Ulsan, Ulsan 44610, Republic of Korea.
School of Materials Science and Engineering, University of Ulsan, Ulsan 44610, Republic of Korea.
Int J Biol Macromol. 2024 May;268(Pt 1):131815. doi: 10.1016/j.ijbiomac.2024.131815. Epub 2024 Apr 24.
We report on the extraction of β-chitin from pens (or Gladius) of Uroteuthis edulis, a squid species prevalent in the Pacific coastal regions of East Asia. In particular, we employ cryogenic mechanical grinding (or cryomilling) as a pre-treatment process for the raw squid pens. We show that the cryomilling step enables an effective pulverization of the raw materials, which facilitates the removal of protein residues allowing the extraction of high-purity β-chitin with a high acetylation degree (∼97 %) and crystallinity (∼82 %). We also demonstrate that the Uroteuthis edulis extract β-chitin affords a free-standing film with excellent optical transmittance and mechanical properties.
我们报告了从太平洋东亚沿海地区普遍存在的乌贼品种（或乌贼属）的笔（或 Gladius）中提取β-壳聚糖的方法。特别是，我们采用低温机械研磨（或低温球磨）作为原始乌贼笔的预处理工艺。我们表明，低温球磨步骤可以有效地粉碎原材料，从而去除蛋白质残留，从而提取出具有高乙酰化度（约 97%）和结晶度（约 82%）的高纯度β-壳聚糖。我们还证明，乌贼属提取的β-壳聚糖可以形成具有优异光学透明度和机械性能的独立膜。
