Lavall Rodrigo L, Assis Odilio B G, Campana-Filho Sérgio P
Instituto de Química de São Carlos, Universidade de São Paulo, Av. Trabalhador são-carlense, 400 - 13560-970 São Carlos/SP, Brazil.
Bioresour Technol. 2007 Sep;98(13):2465-72. doi: 10.1016/j.biortech.2006.09.002. Epub 2006 Oct 25.
Recently the squid pens, a rich source of beta-chitin containing low contents of inorganic compounds, have become available in considerable amounts as a refuse of the fishery industries in Brazil. Thus, the aim of this work is to use squid pens from Loligo sanpaulensis and Loligo plei, species found in the Brazilian coast, as the raw material for the extraction of beta-chitin. The squid pens were submitted to the usual sequence of treatments used for chitin extraction - demineralization and deproteinization - but due to its low content of inorganic compounds a two-step alkaline treatment was enough to produce beta-chitin with low contents of ash (< or = 0.7%). Indeed, the low contents of ash and metals, such as Ca (< or = 10.4 ppm), Mg (< or = 2.5 ppm), Mn (< or = 3.1 ppm) and Fe (< or = 1.8 ppm), are lower than those reported in most of the papers found in the literature. Also, the beta-chitin extracted by employing only the alkaline treatment was more acetylated than the other samples prepared in this work. Regardless of the treatment employed for the extraction of the beta-chitin from the squid pens, its infrared spectra and X-ray diffraction pattern presented only minor differences, however they were clearly distinguished from commercial alpha-chitin.
最近，鱿鱼笔作为一种富含β-几丁质且无机化合物含量低的资源，在巴西渔业中作为废弃物大量可得。因此，本研究的目的是使用来自巴西海岸发现的圣保罗枪乌贼和莱氏枪乌贼的鱿鱼笔作为提取β-几丁质的原料。鱿鱼笔经过了用于几丁质提取的常规处理流程——脱矿质和脱蛋白——但由于其无机化合物含量低，两步碱处理就足以生产出灰分含量低（≤0.7%）的β-几丁质。事实上，灰分和金属（如钙（≤10.4 ppm）、镁（≤2.5 ppm）、锰（≤3.1 ppm）和铁（≤1.8 ppm））的低含量低于文献中大多数论文所报道的含量。此外，仅采用碱处理提取的β-几丁质比本研究中制备的其他样品乙酰化程度更高。无论采用何种处理方法从鱿鱼笔中提取β-几丁质，其红外光谱和X射线衍射图谱仅呈现出微小差异，然而它们与商业α-几丁质明显不同。
