Department of Civil Infrastructure and Environmental Engineering, Khalifa University, PO Box, 127788, Abu Dhabi, United Arab Emirates.
Department of Chemical and Petroleum Engineering and Research and Innovation Center on CO(2) and Hydrogen (RICH Center), Khalifa University, PO Box, 127788, Abu Dhabi, United Arab Emirates.
Chemosphere. 2024 Jun;358:142145. doi: 10.1016/j.chemosphere.2024.142145. Epub 2024 Apr 24.
This research reported on the immobilization of environmentally friendly enzymes, such as horseradish peroxidase (HRP) and laccase (L), along with the hydrophilic zwitterionic compound l-DOPA on nano-filtration (NF) membranes. This approach introduced biocatalytic membranes, leveraging combined effects between membranes and enzymes. The aim was to systematically assess the efficacy of the enzymatic modified membrane (HRP-NF) in degrading colors in the wastewater, as well as enhancing the membrane resistance toward organic fouling. The enzymatic immobilized membrane demonstrated 96.3 ± 1.8% to 96.6 ± 1.9% removal of colors, and 65.2 ± 1.3% to 67.2 ± 1.3% removal of TOC. This result was underpinned by the insights obtained from the radical scavenger coumarin, which was employed to trap and confirm the formation of PRs through the reaction of enzymes and HO. Furthermore, membranes modified with enzymes exhibited significantly improved antifouling properties. The HRP-NF membrane experienced an 8% decline in flux, while the co-immobilized HRP-L-NF membrane demonstrated as low as 6% flux decline, contributed by the synergistic effect of increased hydrophilicity and biocatalytic effects. These findings confirmed that the immobilized enzymatic surface has added function of degrading contaminants in addition to separation function of nanofiltration membrane. These l-DOPA-immobilized enzymatic membranes offered a promising hybrid biocatalytic membrane to eliminate dyes and mitigate membrane fouling, which can be applied in many industrial and domestic water and wastewater treatment.
本研究报告了将环境友好型酶（如辣根过氧化物酶（HRP）和漆酶（L））与亲水性两性离子化合物 l-DOPA 固定在纳滤（NF）膜上。这种方法引入了生物催化膜，利用了膜和酶之间的协同效应。目的是系统评估酶修饰膜（HRP-NF）在降解废水中颜色以及提高膜对有机污染阻力方面的效果。固定化酶的膜显示出 96.3±1.8%至 96.6±1.9%的颜色去除率，以及 65.2±1.3%至 67.2±1.3%的 TOC 去除率。自由基清除剂香豆素的应用证实了这一点，它通过酶和 HO 的反应捕获并确认了 PR 的形成。此外，经过酶修饰的膜表现出显著改善的抗污染性能。HRP-NF 膜的通量下降了 8%，而共固定化 HRP-L-NF 膜的通量下降了低至 6%，这归因于亲水性和生物催化效应的协同作用。这些发现证实，固定化酶表面除了具有纳滤膜的分离功能外，还具有降解污染物的附加功能。这些 l-DOPA 固定化酶膜提供了一种有前途的混合生物催化膜，可用于消除染料和减轻膜污染，可应用于许多工业和家庭用水和废水处理。
