Department of Chemistry, Technology and Biotechnology of Food, Faculty of Chemistry, Advanced Materials Center, Gdańsk University of Technology, G. Narutowicza 11/12 Str., 80-233 Gdansk, Poland.
Department of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdansk University of Technology, G. Narutowicza 11/12 Str., 80-233 Gdansk, Poland.
Carbohydr Polym. 2024 Jul 15;336:122120. doi: 10.1016/j.carbpol.2024.122120. Epub 2024 Apr 2.
This study introduces a method for producing printable, thermosensitive bioink formulated from agarose (AG) and carbon dioxide-saturated chitosan (CS) hydrogels. The research identified medium molecular weight chitosan as optimal for bioink production, with a preferred chitosan hydrogel content of 40-60 %. Rheological analysis reveals the bioink's pseudoplastic behavior and a sol-gel phase transition between 27.0 and 31.5 °C. The MMW chitosan-based bioink showed also the most stable extrusion characteristic. The choice of chitosan for the production of bioink was also based on the assessment of the antimicrobial activity of the polymer as a function of its molecular weight and the degree of deacetylation, noting significant cell reduction rates for E. coli and S. aureus of 1.72 and 0.54 for optimal bioink composition, respectively. Cytotoxicity assessments via MTT and LDH tests confirm the bioink's safety for L929, HaCaT, and 46BR.1 N cell lines. Additionally, XTT proliferation assay proved the stimulating effect of the bioink on the proliferation of 46BR.1 N fibroblasts, comparable to that observed with Fetal Bovine Serum (FBS). FTIR spectroscopy confirms the bioink as a physical polymer blend. In conclusion, the CS/AG bioink demonstrates the promising potential for advanced spatial cell cultures in tissue engineering applications including skin regeneration.
本研究介绍了一种可打印的热敏生物墨水的制备方法，该生物墨水由琼脂糖（AG）和二氧化碳饱和壳聚糖（CS）水凝胶组成。研究确定中分子量壳聚糖是生物墨水生产的最佳选择，生物墨水的最佳壳聚糖水凝胶含量为 40-60%。流变分析表明生物墨水具有假塑性行为和溶胶-凝胶相转变，温度范围在 27.0 和 31.5°C 之间。MWM 壳聚糖基生物墨水还表现出最稳定的挤出特性。选择壳聚糖来生产生物墨水也是基于评估聚合物的抗菌活性与其分子量和脱乙酰度的关系，对于最佳生物墨水组成，壳聚糖对大肠杆菌和金黄色葡萄球菌的细胞减少率分别为 1.72 和 0.54。通过 MTT 和 LDH 测试进行的细胞毒性评估证实了生物墨水对 L929、HaCaT 和 46BR.1N 细胞系的安全性。此外，XTT 增殖试验证明了生物墨水对 46BR.1N 成纤维细胞增殖的刺激作用，与胎牛血清（FBS）观察到的作用相当。傅里叶变换红外光谱（FTIR）证实了生物墨水是一种物理聚合物共混物。总之，CS/AG 生物墨水具有在组织工程应用中进行高级空间细胞培养的潜力，包括皮肤再生。
